A flow inj ection analysis(FIA)system for determination of adenosine triphosphate(ATP)was de-veloped.The polyion-sensitive membrane electrodes were used for potentiometric detection and the aptamers were used for molecular recognition.The electrostatic interaction between aptamer and protamine could cause potential response on the electrodes.Interaction between aptamer and ATP changed the forms of aptamer,which prevented the interaction between aptamer and protamine.Based on that,the concentration of ATP could be de-termined by potential detection.Results showed that,under optimized conditions(current of 1 0 nA,flow rate of 2.0 mL·min-1 ,sample volume of 80μL),the proposed method showed a good linear relationship to ATP in the concentration range from 2.0 to 12.0μmol·L-1 .The detection limit was calculated to be 1.4μmol·L-1 . This method can also be used for the determination of other targets.%以核酸适体为识别分子、以经鱼精蛋白活化的聚离子敏感膜电极为信号识别单元，构建了一种检测三磷酸腺苷（ATP）的流动注射分析方法。核酸适体与鱼精蛋白之间的静电作用能够引起电极电位的变化，核酸适体与 ATP的相互作用使核酸适体的形态发生一定的变化，这种变化能够阻止核酸适体与鱼精蛋白之间的作用，据此实现对 ATP的电位检测。结果表明，在电流为10 nA、载液流速为2.0 mL·min-1、进样量为80μL的优化条件下，本方法对ATP检测的线性范围为2.0～12.0μmol· L-1，检出限为1.4μmol· L-1。本方法亦可用于对其它聚阴离子检测。
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