首页> 中文期刊> 《化学与生物工程》 >南极微生物絮凝剂产生菌的筛选、分子鉴定及适应性研究

南极微生物絮凝剂产生菌的筛选、分子鉴定及适应性研究

         

摘要

Seven microbial flocculant-producing bacteria were separated from antarctic seawater,sea ice and marine sediment. The molecular identification of these bacteria was by the way of 16S rRNA gene sequence amplification. Phylogenetic analysis showed that they belonged to Acinetobacter,Arthrobacter,Psychrobacter,Ser-ratia,Pseudomonas and Pseudoalteromonas, respectively. Different carbon sources and nitrogen sources were used to culture the bacteria in order to observe the change of flocculation activity. The bacteria, 1-2 and P2-13-1, were of universal adaptability to different carbon sources and nitrogen sources and had higher flocculation activity in economic culture medium.%从南极海水、海冰、底泥中分离得到7株产絮凝剂的菌株,通过16S rRNA基因序列扩增方法进行分子鉴定,分别隶属于不动杆菌属(Acinetobacter)、节杆菌属(Arthrobacter)、嗜冷杆菌属(Psychrobacter)、沙雷菌属(Serratia)、假单胞菌属(Pseudomonas)和假交替单胞菌属(Pseudoa lteromonas).改变培养基中碳源和氮源的组成并观察絮凝率的变化,筛选出在经济培养基条件下具有较高絮凝活性、同时对碳源和氮源具有普适性的菌株I-2和P2-13-1.

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