为摸清靖边县奶牛布鲁氏菌病流行情况,2017年7—8月对该县全部存栏奶牛进行了布鲁氏菌病血清学调查.共采集98个奶牛养殖场户的1227份血清样品,分别用虎红平板凝集试验(RBPT)、试管凝集试验(SAT)和竞争酶联免疫吸附试验(C-ELISA)3种血清学检测方法,对其进行布鲁氏菌血清抗体检测.结果显示:奶牛布鲁氏菌病群体阳性率为13.27％,个体阳性率为3.50％,其中散养户个体阳性率较高,为7.52％,远高于我国控制区标准,需重点加强防控.为探讨上述3种检测方法的一致性,对3种检测方法的检测结果进行了对比分析.结果显示:RBPT、SAT和C-ELISA 3种方法检测结果的一致率大于99％,Kappa值大于0.90,表明3种方法具有良好的一致性.因此,在奶牛布鲁氏菌病监测净化过程中,将3种血清学检测方法联合应用,能有效降低出现假阳性和假阴性的概率,准确识别野毒感染牛,从而减少阳性牛处理过程中的误杀和漏杀现象.%In order to recognize the prevalence status of cow Brucellosis in Jingbian County of Shaanxi Province,a serological investigation was carried out during July to August of 2017. A total of 1227 serum samples were collected from 98 dairy farms and households and detected by three serological methods of the rose bengal plate agglutination test(RBPT),tube agglutination test(SAT)and competitive enzyme-linked immunosorbent assay(C-ELISA). The results showed that the group positive rate of Brucellosis was 13.27％,the individual positive rate was 3.50％. Among them,the individual positive rate of backyard households was 7.52％,which was higher than the standard of control area in China. In order to discuss the consistency of the above three detection methods,the results of the three detection methods were compared and analyzed. Results showed that the agreement rate of the three methods of RBPT,SAT and C-ELISA was higher than 99％,and theKappavalue was larger than 0.90,indicating a good consistency. Therefore, during the process of surveillance and purification of cow Brucellosis,combined application of the three detection methods could effectively reduce the probability of false positives and negatives,accurately identify the cow infected with wild strains,so as to reduce the mistaken and omissive slaughtering.
展开▼
