In this study, Trichodcrma reesei Rut C-40 was inoculated to the steam-explosion com stalks to produced cellulase. Then the yeasts were cultivated in the mixture of cellulase and steam-explosion stalk to produce ethanol. The optimal conditions for enzymatic hydrolysis were determined by Box-Behnken, which were the proportion of cellulase to steam-explosion stalk was 1.2, temperature 46℃ and pH value 4.4. Hydrolysis yield was 32.50% after 48h saccharification. When the yeasts were inoculated to the substrates, the productivity of ethanol was 0.15g/g substrate after 96h, which was 9.3% higher than the yield of SSF without saccharification.%该文以玉米秸秆为原料,经蒸汽爆破预处理后接入Trichoderma reesei Rut C-40培养纤维素酶曲,将纤维素酶曲与汽爆秸秆混合堆积糖化后,接入酵母菌进行同步糖化固态发醇生产乙醇,通过Box-Behnken设计实验得到最适酶解工艺条件:酶曲/汽爆秸秆为1.2,温度46℃,pH值4.4,堆积糖化48h后酶解率可达到32.50％.将酶解糖化48h后的底物接入酵母菌,发酵96h后乙醇产率可达0.15g/g底物,较直接同步糖化发酵乙醇产率提高了9.3％.
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