A sensitive liquid chromatographic mcthod for the detection of andno acids with precolumn fluorescence derivatization with acridone-N-acetyl chloride has been developed. Andno acid derivatives were eluted in 4o ndn with good reproducibility. The reIative standard deviations (n=6) at an analytical concentration of 50 pmol are < 4%. The method described is also suitable for the analysis of andno acids in different biological samples.
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机译:simple derivatization method for sensitive determination of fatty acids with fluorescence detection by high-performance liquid chromatography using 9-(2-hydroxyethyl)-carbazole as derivatization reagent