Objective To investigate the relationship between interleukin-1 (IL-1), tumor necrosis factor (TNF) gene polymorphism and coronary heart disease. Methods 120 cases of patients with coronary heart disease in our hospital were included in the study group. 120 healthy subjects were included in the control group. The clinical data of two groups were collected. Polymerase chain reaction (PCR) method was used for the determination of interleukin-1 receptor antagonist gene (IL-1RN ) type. Restriction fragment length polymorphism (RFLP) method was used to analyze TNF gene and IL-1B gene polymorphism. The correlation with coronary heart disease was studied. Results There was no difference in the proportions of IL-1B-31 and IL-1RN loci between different genotypes (P>0.05). The proportion of IL-1B-511 loci of T/T genotype in the study group was higher than that in the control group (P<0.05), and the risk for coronary heart disease of T/T and C/T genotype was higher than that of C/C genotype. The proportion of TNF-a-863 loci of C/C genotype in the study group type was higher than that in the control group, while that of C/A genotype was lower than that in the control group (P<0.05). The risk for coronary heart disease of C/A genotype was the highest. Conclusion IL-1B-511 T/T and TNF-a-863 are the independent risk factors for coronary heart disease.%目的：探讨白细胞介素-1（IL-1）、肿瘤坏死因子（TNF）基因多态性与冠心病的关系。方法选取我院收治的120例冠心病患者作为研究组，120例健康体检者作为对照组，收集两组患者的临床资料，采用聚合酶链反应（PCR）法测定白细胞介素-1受体拮抗剂基因（IL-1RN）型，用PCR限制性片段长度多态性（RFLP）法分析TNF基因、IL-1B基因多态性，研究其与冠心病相关性。结果两组IL-1B-31、IL-1RN位点各基因型所占比例对比差异无统计学意义（P＞0.05）；IL-1B-511位点研究组T/T型所占比例高于对照组（P＜0.05），且T/T、C/T基因型冠心病发病风险均高于C/C型；TNF-a-863位点研究组C/C型所占比例高于对照组，C/A型低于对照组，对比差异有统计学意义（P＜0.05），其中C/A型冠心病发病风险最高。结论IL-1B-511 T/T、TNF-a-863均为冠心病的独立危险因素。
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