通过批次实验,分别研究了丙氨酸对于厌氧氨氧化过程的短期以及长期影响.研究表明,当丙氨酸为唯一底物时,无论短期还是长期培养,厌氧氨氧化过程均受到很大影响,体系内未发生氮的去除.在缺乏电子受体NO2--N的情况下,体系内未发生厌氧氨氧化反应,虽然丙氨酸降解率达到了86%以上,但产生的NH4+-N在体系内积累.当向丙氨酸为底物的系统中投加NH4+-N和NO2--N时,短期(7h)培养中厌氧氨氧化活性受到的影响较小.同时,2mmol/L丙氨酸在厌氧氨氧化体系中10h即可去除78%,高浓度(10mmol/L)丙氨酸在60h达到99%的去除率;长期培养过程中,浓度为2mmol/L的丙氨酸一定程度上抑制了厌氧氨氧化活性.在厌氧氨氧化与反硝化的共同作用下,TN的去除率达到57%,丙氨酸的去除率为99%左右.%The short-term and long-term effects of alanine on ANAMMOX process were investigated by batch experiments. The ANAMMOX process was greatly affected both in short-term and long-term culture when alanine was the sole substrate, and there was no nitrogen removal in the system. Without electron acceptor NO2--N, ANAMMOX process could not occur in the system, although the alanine removal could reach more than 86%, NH4+-N was accumulated in the system. When NH4+-N and NO2--N were added to the system with alanine as a substrate, the activity of ANAMMOX bacteria was not affected so much in the short term (7h), and 2mmol/L and 10mmol/L alanine reached 78% and 99% removal efficiencies in 10 and 60h, respectively. During the long term experiments, the activity of ANAMMOX bacteria could be inhibited by alanine with a concentration of 2mmol/L. Combined with ANAMMOX and denitrification processes, the removal efficiency of TN reached 57%, and the removal efficiency of alanine was about 99%.
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