目的：分析研究酶联免疫吸附实验(ELISA)与免疫胶体金法在梅毒检测中各自的优劣和适用性。方法选取该院门诊及住院患者8600例,采取静脉血液5 mL,待血清析出后,3000 r/min离心5 min,取血清分析；所有患者均先用ELISA法进行梅毒检测,再用免疫胶体金法复检；比较两种方法检测出的阳性数、阴性数。结果8600例患者经ELISA检测,阳性155例(1.8%)；经免疫胶体金法检测,阳性105例(1.22%)；两种梅毒检测方法结果比较2=9.763,P<0.01,差异具有统计学意义。结论梅毒检测中ELISA与免疫胶体金法比较,ELISA阳性检出率更高。同时,为确保梅毒确诊率,还应充分结合患者各方面资料以及其他检测方法综合判断。%Objective Analysis of enzyme linked immunosorbent assay (ELISA) and immune colloidal gold method in detection of Treponema pallidum their advantages and disadvantages and applicability. Methods To choose 8 600 patients of the outpatient and inpatient, taking venous blood for serum 5mL, precipitation, 3 000 r/min centrifugal 5min, serum analysis;all patients were tested for syphilis by ELISA method at first, and then the immune colloidal gold method re examination;positive numbers, negative detected to compare the two methods of number.Results 8 600 patients were detected by ELISA, 155 cases were positive (1.8%);the detection of immune colloidal gold method, 105 cases were positive (1.22%);two syphilis detection method compared the results of c2=9.763, p<0.01, with significant difference. Conclusion Comparison of ELISA and colloidal gold method in detection of syphilis, ELISA positive rate is higher. At the same time, to ensure that the syphilis diagnosis rate, also should be fully integrated with all aspects of patient data and other detection method of comprehensive judgment.
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