Objective: To assess the role of single nucleotide polymorphism (SNP) in promoter region of interleukin- 20 ( IL - 20) in the development of psoriasis and exacerbation by infection.Methods: Luciferas assay was conducted with PGL4.15 - basic vector including patient - derived or artificially mutated IL- 20 promoter region.HaCat cells were transfected with the conducted vector and stimulated by LPS, CpG - ODNs or IL- 1β.Results: Luciferase reporter assays showed that the risk - associated G allele of promoter region - 1723 led to significantly higher promoter activity than non- risk C allele ( P ＜ 0.01 ).Furthermore, the G allele brought about stronger promoter activity after stimulation of IL- 1β and CpG - ODNs than before, while the C allele did not have significant difference.But these two alleles of rs1713239 had no response under stimulation of LPS.Conclusion: This study has demonstrated that transcriptional modulation of IL- 20 expression by SNP has an impact on the development of psoriasis and exacerbation by infection.%目的:评价IL-20基因启动子区-1723位点单核苷酸多态性在银屑病发生及感染后加重中的作用.方法:构建携带该位点不同等位基因的荧光素酶报告基因,转染人永生化角质形成细胞系(HaCaT细胞),LPS,CpG-ODNs及IL-1β刺激后,检测报告基因的荧光强度.结果:①该位点携带不同等位基因的荧光素酶报告基因的荧光强度有统计学差异(P<0.01).②在IL-1β和CpG-B刺激下,携带等位基因G的荧光强度较刺激前明显增加(分别P<0.01),携带C等位基因的荧光强度在刺激前后无明显变化(P>0.05).LPS刺激后两个等位基因的荧光强度均无统计学差异(P>0.05).结论:IL-20-1723位点单核苷酸多态性通过调节IL-20启动子区转录活性来影响IL-20的表达,其与感染在银屑病的发病中有着协同作用.
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