目的:探讨50 Hz 1.8 mT正弦交变电磁场(sinusoidal electromagnetic fields,SEMFs)对SD大鼠骨密度(bone mineral density,BMD)的影响.方法:将30只1月龄体重为(110±10)g的SD大鼠随机分为正常对照组和电磁场组,各15只.分别给予对照组50 Hz 0 mT和电磁场组50 Hz 1.8 mT强度正弦交变电磁场干预,1.5 h/d,每周称取体重1次,每天监测进食量变化,6周后腹腔注射麻醉大鼠并用双能X骨密度检测全身骨密度,处死后检测股骨骨密度及椎体骨密度;ELISA法测定血清骨钙素(Osteocalcin,OC)和血清抗酒石酸性磷酸酶5b (tartrate-resistant acid phosphatase 5b,TRACP 5b);剥离肝、肾、子宫称重,计算器官指数并做HE切片做常规病理学检测分析.结果:与对照组相比,电磁场组大鼠各周体重、每日进食量未见明显变化;2、4周后全身骨密度无明显变化,6周后全身骨密度及离体股骨和椎骨骨密度显著增加;血清OC表达量显著增加,血清TRACP 5b表达量显著降低;子宫、肝脏、脾脏HE染色未见病变,脏器指数无异常.结论:50 Hz 1.8 mT正弦交变电磁场能通过提高骨形成降低骨吸收相关因子的表达,从而提高青年大鼠的峰值骨密度,为临床电磁场预防骨质疏松的研究奠定基础.%Objective:To study effects of 50 Hz 1.8 mT sinusoidal electromagnetic fields (SEMFs) on bone mineral density (BMD) in SD rats.Methods:Thirty SD rats weighted (110±10) and aged 1 month were randomly divided into control group and electromagnetic field group,15 in each group.Normal control group of 50 Hz 0 mT density and sinusoidal electromagnetic field group of 50 Hz 1.8 mT were performed respectively with 1.5 h/d and weighted weight once a week,and observed food-intake.Rats were anesthesia by intraperitoneal injection and dual energy X-ray absorptiometry were used to detect bone density of whole body,and detected bone density of femur and vertebral body.Osteocalcin and tartrate-resistant acid phos phatase 5b were detected by ELSA;weighted liver,kidney and uterus to calculate purtenance index,then detected pathologic results by HE.Results:Compared with control group,there was no significant change in weight every week,food-intake every day;no obvious change of bone density of whole body at 2 and 4 weeks,however bone density of whole body,bone density of excised femur and vertebra were increased at 6 weeks.Expression of OC was increased,and TRACP 5b expression was decreased.No change of HE has been observed in liver,kidney and uterus and organic index.Conclusion:50 Hz 1.8 mT sinu soidal electromagnetic fields could improve bone formation to decrease relevant factors of bone absorbs,to improve peak bone density of young rats,in further provide a basis for clinical research electromagnetic fields preventing osteoporosis foundation.
展开▼
