目的:分析和解决高效液相色谱在使用过程中出现的多种技术问题。方法:总结高效液相色谱技术在使用过程常发生的柱压力不稳、基线噪音增大、色谱柱柱效下降以及化合物保留时间漂移等问题,提出了简单有效的解决方法。结果:通过分析判断,解决了高效液相色谱的技术问题,有效提高了仪器完好率和使用率,保证科学研究和教学工作的正常进行。结论:高效液相色谱仪的正确使用、保养和维护可以延长仪器的使用寿命,发挥仪器设备的最大效益。%Objective: To solve the difficulties and problems in the process of using high performance liquid chromatography. Methods:Summarizing the problems often occurred in this technology and providing some simple and effective solutions. Results:The instrument intact rate and utilization rate have been improved, which guarantees the scientific research and teaching. Conclusion:Proper maintenance of high performance liquid chromatography can prolong their service lives, and give play to their greatest benefits.
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