Objective:To explore the effect of Simvastatin on bone mineral density (BMD) and bone metabolism factors of ovariectomized mice.Methods:36 female Kunming mice who were unpregnancy and cleaning grade were selected in this research.Post-menopausal osteoporosis mice models with high-transformation were created by ovariectomy.All of them were divided into two groups:model group (25 mice) and sham operation group (11 mice) by randomization.After 30 days post-operation, 20 survival mice of model group were randomly divided into model group (10 mice) and intervention group (10 mice) .And there were 10 mice survived in sham group.The model group and the sham group were implemented normal saline (NS) , and the intervention group was implemented Simvastatin suspension.The vaginal smear method was used to determine whether the ovaries of mice were completely removed.And enzyme-linked immunosorbent assay (ELISA) method was adopted to detect bone alkaline phosphatase (BALP) , tartrate-resistant acid phosphatase 5b (TRAP5b) and bone glaprotein (BGP) in serum of mice.Results:After ovaries of mice were removed, the BMD of model group was significantly lower than that of sham group (t=0.00015, P<0.05) .After the intervention treatment with Simvastatin was implemented, the concentrations of serum BALP, TRAP5b and BGP and BMD of three groups were significantly enhanced.And the levels of serum BALP, TRAP5b and BGP were significantly down-regulated, and the differences among three groups were significantly.Conclusion:Simvastatin has obviously therapeutic effects on osteoporosis with high-transformation, that causes by ovariectomy, of mice.%目的:探索辛伐他汀对去卵巢小鼠骨密度 (BMD) 及骨代谢因子的影响.方法:选取36只清洁级未孕雌性昆明小鼠, 通过对小鼠行卵巢摘除手术, 制作绝经后高转换骨质疏松小鼠模型.采用随机分组法将小鼠分为模型组 (25只) 和假手术组 (11只) ;术后30 d, 将模型组存活的20只小鼠随机分为模型组 (10只) 和干预组 (10只) ;假手术组存活10只.模型组和假手术组给予生理盐水, 干预组给予辛伐他汀混悬液, 均连续灌胃给药12周.对小鼠阴道涂片后检测卵巢摘除是否完全;采用酶联免疫法检测血清中骨性碱性磷酸酶 (BALP) 、抗酒石酸酸性磷酸酶 (TRAP5b) 和骨钙素 (BGP) 含量.结果:卵巢摘除后, 模型组与假手术组小鼠的BMD下降比较差异有统计学意义 (t=0.00015, P<0.01) ;给予辛伐他汀干预治疗后, 3组血清中的BALP、TRAP5b和BGP含量和BMD均显著升高;血清中的BALP、TRAP5b和BGP含量均明显下调, 三组比较具有显著差异.结论:辛伐他汀对小鼠卵巢摘除导致的高转换型骨质疏松具有明显的治疗作用.
展开▼
