目的 探讨壮骨止痛方对去卵巢骨质疏松大鼠血清降钙素基因相关肽(CGRP)及骨组织β1-AR表达的影响.方法 72只SPF级雌性SD大鼠随机选出12只,摘除卵巢周围相应质量脂肪后作为假手术组.其余60只采用双侧去卵巢法制备骨质疏松病理模型,随机分为模型组、壮骨止痛方高剂量组(13.2 g/kg)、中剂量组(6.6 g/kg)、低剂量组(3.3 g/kg)和戊酸雌二醇组(2.1×10-5 g/kg),每组各12只.模型组及假手术组给予等体积纯净水,均干预13周.采用ELISA法检测血清CGRP含量,免疫组化法检测骨组织β2-AR蛋白表达.结果 与假手术组比较,模型组大鼠血清CGRP含量显著减少,骨组织β2-AR蛋白表达显著下调,差异均有高度统计学意义(P＜0.01).与模型组比较,戊酸雌二醇组及壮骨止痛方各剂量组大鼠血清CGRP含量显著增加,骨组织β1-AR蛋白表达上调,差异均有统计学意义(P＜ 0.05或P＜ 0.01).壮骨止痛方不同剂量组组间比较,差异无统计学意义(P＞0.05).结论 壮骨止痛方能够促进去卵巢骨质疏松模型大鼠CGRP表达,上调骨组织β2-AR蛋白表达,这可能是其抗骨质疏松的作用机制之一.%Objective To explore the effect of Zhuanggu Zhitong prescription on the CGRP and β2-AR in ovariectomized osteoporosis rats and investigate its mechanism in anti-osteoporosis.Method 72 rats were randomly divided into sham operation group (12 cases) and model group (60 cases),the model group rats were ovariectomized osteoporosis and the sham operation group rats were excised equal weight fat.After the modeling,the rats were randomly divided into model group,Zhuanggu Zhitong prescription high-dose group (13.2 g/kg),middle-dose group (6.6 g/kg),low-dose group (3.3 g/kg) and estradiol valerate group (2.1×10-5 g/kg),with 12 rats in each group.The model group and sham operation group rats were given equal volume water.All intervened for 13 weeks.The content of CGRP in serum was detected by ELISA and protein expression of β2-AR in bone tissue were detected by immunohistochemistry.Results Compared with sham operation group,the content of CGRP in serum and protein expression of β2-AR in bone tissue of model group were significantly declined,with high statistically significant differences (all P ＜ 0.01).Compared with the model group,the content of CGRP in serum and protein expression of β2-AR in bone tissue of different doses Zhuanggu Zhitong prescription group and estradiol valerate group were increased significantly,with statistically significant differences (P ＜ 0.05 or P ＜ 0.01).There were no significant differences in different doses of Zhuanggu Zhitong prescrip tion groups (P ＞ 0.05).Conclusion Zhuanggu Zhitong prescription can promote the secretion of CGRP and protein expression of β2-AR,which may be one mechanism of ZhuangguZhitong prescription to antiosteoporosis.
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