目的 探讨柑橘素预处理对大鼠离体心脏缺血/再灌注损伤的保护作用.方法 56只SD雄性大鼠,随机分为正常对照组(CON)、柑橘素1、2.5、5、10、20、40 μmol/L组(NARI).各组大鼠心脏平衡灌流30 min后,对照组给予30 min全心缺血和60 min再灌注处理(或120 min用于测定心肌梗死面积),NARI组大鼠缺血前分别给予1、2.5、5、10、20、40 μmol/L Nari灌流10 min预处理,再行30 min全心缺血和60 min再灌注处理(或120 min用于测定心肌梗死面积).观测各组大鼠左心室舒缩功能,测定心肌乳酸脱氢酶含量、心肌超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,并计算心肌梗死面积百分比.结果 与CON组相比,2.5 μmol/L及以上浓度NARI预处理组大鼠缺血/再灌注后心功能恢复提高、乳酸脱氢酶含量降低和心肌梗死面积减少,差异有统计学意义(P＜ 0.05或P＜ 0.01).与对照组相比,2.5 μmol/L及以上浓度NARI组大鼠心肌SOD活力明显增加,MDA含量明显降低,差异有统计学意义(P＜ 0.05或P＜0.01).结论 柑橘素对大鼠离体心脏缺血/再灌注损伤具有保护作用,其作用机制可能与增强心肌对缺氧的适应能力相关.%Objective To investigate the protective effect of naringenin on myocardial ischemia/reperfusion injury in isolated rat hearts.Methods 56 SD rats were randomly divided into:normal control (CON) group,naringenin (NARI) of 1,2.5,5,10,20,40 μmol/L groups.After balance perfusion for 30 min in each group,CON group was treated with 30 min global ischemia followed by 60 min reperfusion (or 120 min for determination of the infarct size).The NARI rats hearts were pretreated with 1,2.5,5,10,20,40 μmol/L Nari perfusion for 10 min before ischemia,and then 30 min global ischemia and 60 min reperfusion (or 120 min for determine the infarct size).The left ventricular function was obseved;levels of myocardial lactate dehydrogenase,myocardial superoxide dismutase (SOD) activity and malondialdehyde (MDA) were measured;and the percentage of myocardial infarct size was calculated in each group.Results Compared with the CON group,the recovery of heart function was increased,the content of lactate dehydrogenase and the area of myocardial infarction were decreased after ischemia/reperfusion in 2.5 μmol/L and above NARI pretreatment group (P ＜ 0.05 or P ＜ 0.01).Compared with the control group,the SOD activity of myocardium significantly increased and the content of MDA significantly decreased in 2.5 μmol/L and above NARI pretreatment group (P ＜ 0.05 or P ＜ 0.01).Conclusion Naringenin has protective effect for isolated rats hearts with ischemia/reperfusion injury,its mechanism may be associated with enhanced myocardial antioxidant capacity.
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