The optimization of parameters is the most important in ultrasound-targeted microbuhble destruction (UTMD),this parameter should achieve both high transfection efficiency while at the same time most of the cells without damage.At present,many studies have focused on optimizing the transfection parameters for those with high transfection efficiency and small tissue damage.However,there are many factors that affect the transfection effect,such asultrasound intensity,duty cycle,irradiation time,microbubble concentration,microbubble properties,plasmid concentration,different cell types.This article reviewes the best parameters of gene transfection in UTMD,and expectes to provide a basis for UTMD to be better applied to gene therapy.%运用超声靶向破坏微泡定位释放技术(UTMD)最重要的是参数的优化,应满足既可以获得高的转染效率的同时又对大部分细胞没有损害.目前许多研究都致力于将转染率高、组织损伤小的转染参数进行优化,但影响转染效果的因素有很多,如超声强度,占空比,辐照时间,微泡浓度,微泡性质,质粒浓度,不同细胞种类等,本文就目前UTMD技术促基因转染最佳参数进行综述,期望为UTMD更好的应用于基因治疗提供研究基础.
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