Objective To compare the transdermal drug delivery in v itro of Dracorhodin in Liubaibaibi Cataplasm be-tween the dragonis ultramicro powder and common powder and provide a reference for the preparation of the cataplasm. Methods The drug transdermal diffusion experiments instrument was used, the chromatographic conditions were as fol-lows: a Diamonsil C18 (250 mmí4.6 mm, 5 μm) with acetonitrile-sodium dihydrogen phosphate anhydrous (0.05 mol/L) (50:50) as mobile phase, the detecting wavelength was 440 nm, and the column temperature was 40℃. The flow rate was 1.0 mL/min. The situation of 12 h in vitro transdermal permeation of dracorhodin in rabbits was detected in two cataplasm samples. Results The dracorhodin peak separation was good, the RSD of the stability, accuracy, recovery were 1.14%, 0.92%, 0.89%, which conformed to the requirements of determination. For both of the samples, the trans-dermal permeation were rapid in 1-2 h, and the transdermal permeation were stable and slow in 2-12 h, the dra-corhodin accumulative transdermal delivery rate of ultra-fine powder cataplasm sample was 29.12% in2 h, 38.60%in 4 h and 59.36% in 12 h, while the common powder cataplasm sample was 16.74% in 2 h, 21.77% in 4 h and 44.28% in 12 h. The dracorhodin accumulative transdermal delivery rate of ultra-fine powder cataplasm sample was 15% higher than that of common powder cataplasm sample in the study of 12 h. Conclusion The method is good, with the superfine powder of dragonis as the cataplasm filler, the transdermal drug delivery in v itro of dracorhodin is significantly superior to the common powder cataplasm sample.%目的：对比研究血竭超微细粉和普通细粉在六白白疕巴布剂中主要药效成分血竭素的体外透皮释药情况，为该巴布剂制备工艺研究提供参考依据。方法使用药物透皮扩散实验仪，以色谱柱为Diamonsil C18柱(250 mm×4.6 mm，5μm)、流动相为乙腈原磷酸二氢钠溶液(0.05 mol/L)(50：50)、检测波长440 nm、柱温40℃、流速1 mL/min的高效液相色谱法(HPLC)色谱条件，检测两种巴布剂样品中血竭素12 h内透过离体兔皮释药情况。结果HPLC检测方法显示血竭素峰分离效果良好，稳定性、精密度和加样回收试验的RSD分别为1.14%、0.92%、0.89%，符合含量测定要求；两种样品巴布剂1~2 h内均为快速透皮释药阶段，2~12 h为平稳缓慢透皮释药阶段。其中超细粉样品巴布剂2、4、12 h内血竭素累积透皮释药率分别为29.12%、38.60%和59.36%，而普通细粉样品巴布剂在同一时间段仅为16.74%、21.77%和44.28%。在考察的12 h内，超微细粉样品巴布剂血竭素累积透皮释药率高于普通细粉样品巴布剂15%左右。结论本含量测定方法良好，以血竭药物超微细粉作为该巴布剂的填充剂，血竭素体外透皮释药效果明显优于以普通细粉作为巴布剂填充剂的样品巴布剂。
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