目的 观察倒卵叶五加及其复方对亚急性衰老大鼠血清丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)以及睾丸组织中NO、iNOS含量的影响.方法 60只雄性SD大鼠随机分为正常对照组、模型组、倒卵叶五加组、佳蓉片组和维生素E组,每组12只.D-半乳糖连续腹腔注射建立亚急性衰老大鼠模型,各组大鼠在腹腔注射D-半乳糖(正常组动物注射等量生理盐水)的同时,正常和模型组大鼠灌胃生理盐水[10 mL/(kg·d)]、其他各组依次给予倒卵叶五加[1 g/(kg·d)]、佳蓉片[6 g/(kg·d)]、维生素E[20 mg/(kg·d)],用药50 d.分别检测各组大鼠血清MDA含量、GSH-Px活性、SOD活性及T-AOC.结果 ①与正常对照组大鼠比较,模型组大鼠MDA含量明显升高,GSH-Px活性、SOD活性、T-AOC明显降低(P < 0.01或P < 0.05);睾丸组织NO和iNOS含量明显升高(P < 0.01).②与模型组比较,倒卵叶五加、佳蓉片、维生素E三组大鼠血清MDA、睾丸组织NO和iNOS含量明显降低(P < 0.01或P < 0.05),GSH-P活性、SOD活性、T-AOC明显升高(P < 0.01).结论 倒卵叶五加和佳蓉片可升高衰老大鼠的抗氧化能力,具有明显延缓衰老的作用;机制可能与其抗氧化和降低衰老大鼠睾丸组织中NO和iNOS的含量有关.%Objective To observe the effect of Acanthopanax obovatus Hoo and its compound preparation on malondi-aldehyde (MDA) content,glutathione peroxidase (GSH-Px) activity,superoxide dismutase (SOD) activity,the total an-tioxidant capacity in serum and NO content,iNOS content in testis of sub-acutely aging rats. Methods 60 male SD rat were randomly divided into 5 groups: normal control group,model group,Acanthopanax obouatus Hoo (AOH) group,Jiarong Tablet group and Vitamin E group,12 rats in each group. The subacute senile rats model was made by in-traperitoneal injection (ip) of D-gal continuous. Each group was dealt with D-gal except the normal group which were injected with isodose physiological saline,meanwhile the normal control group and the model group were lavaged with saline [10 mL/(kg·d)],the other group were lavaged with AOH[1 g/(kg·d)],Jiarong Tablet [6 g/(kg·d)] and Vitamin E [20 mg/(kg·d)] respectively for 50 days. The MDA content,GSH-Px activity,SOD activity,and T-AOC in serum of rats in each group were respectively detected. Results Compared with rats in normal control group ,MDA content increased obviously,GSH-Px activity,SOD activity and T-AOC of rats in model group decreased obviously (P < 0.01 or P < 0.05 )the NO content and the iNOS content in testis increased obviously (P < 0.01). Compared with rats in model group ,the MDA content in serum,the NO content and i NOS content in testis decreased significantly,GSH-Px activity,SOD activity and T-AOC of rats in AHO group,Jiarong Tablet group,Vitamin E increased significantly (P < 0.01). Conclusion AOH and Jiarong Tablet can inhibit declining of anti-oxidative ability of subacute senile rats,has obviously anti-aging effects,the mechanism maybe related with its ability of antioxidant and the effects of reducing NO,i-NOS content in testis of rats.
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