Objective: To establish a method for determination of polysaccharide in Ganoderma Lucidum Paste. Methods: Sulfuric acid-anthrone colorimetry, glucose were as reference standard, measured by spectrophotometer in Ganoderma Lucidum Paste, and studied the determination by the follow aspects: the preparation conditions (petroleum ether extraction, alcohol precipitation time, the number of alcohol precipitation) and the colored condition (wave length, temperature, time). Results: Polysacchatide content of three batches of samples were 2.52％, 2.41％, 2.56％, 2.51％, 2.49％, 2.50％ with aRSD of 1.98％. Conclusion: The method could be accurate and effective in the determination of polysaccharide in Ganoderma Lucidum Paste, and simple, stable, reproducible, and can be used to determinate polysaccharides in Ganoderma Lucidum Paste.%目的:确立灵芝膏中多糖含量测定方法.方法:采用硫酸-蒽酮比色法,葡萄糖作对照品,用分光光度计测量灵芝膏中多糖含量;并从制备条件(石油醚萃取与否、醇沉时间、醇沉次数),显色条件(波长、温度、时间)方面对测定方案进行研究.结果:6批灵芝膏中多糖含量为2.52%、2.41%、2.56%、2.51%、2.49%、2.50%,相对标准偏差(RSD)为1.98%.结论:该法能准确有效地测定出灵芝膏中多糖含量,且操作简单、稳定性、重现性好,可用于本制剂多糖含量的检测.
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