目的 研究多发性骨髓瘤(MM)患者骨髓组织中微小RNA-181a(miRNA-181a)与miRNA-373的表达,探讨其临床意义.方法 选取2015年3月～2017年3月浙江省台州市第一人民医院收治的67例MM 患者作为病例组,选取同期患其他血液疾病的40例患者作为病例对照组、40例健康体检人员作为健康对照组.采用实时荧光定量聚合酶链式反应(qRT-PCR)检测各组骨髓组织中miRNA-181a及miRNA-373的表达,分析各组间两者的表达差异及其与MM 临床病理特征的关系.Pearson 线性相关分析分析miRNA-181a 与miRNA-373 表达的相关性.结果 分别与病例对照组及健康对照组比较,病例组miRNA-181a 表达明显较高,miRNA-373 表达明显较低(P ＜ 0.05),而病例对照组与健康对照组miRNA-181a、miRNA-373 表达差异无统计学意义(P ＞ 0.05).病例组患者骨髓组织中miRNA-181a、miRNA-373 表达与肿瘤分期、肿瘤分化有关(均P ＜ 0.05),其与年龄、性别、细胞学分型及淋巴结转移无关(均P ＞ 0.05).病例组骨髓组织中miRNA-181a 与miRNA-373 表达呈明显负相关(r = -0.696,P ＜ 0.05).病例对照组和健康对照组的miRNA-181a 与miRNA-373 表达无相关性(r = 0.151、0.179,均 P ＞ 0.05).结论 MM 骨髓组织中miRNA-181a 表达升高,而miRNA-373 表达降低,两者共同参与肿瘤的发生、发展过程,可能成为新的MM 诊断、治疗及预后评估的标志物.%Objective To investigate the expression of microRNA-181a (miRNA-181a) and miRNA-373 in bone marrow of patients with multiple myeloma (MM) and to explore its clinical significance. Methods A total of 67 patients with MM who were treated in Taizhou First People's Hospital from March 2015 to March 2017 were selected as the case group, 40 patients with other blood diseases in the same period were selected as the case control group and 40 healthy people were selected as the healthy control group. Quantitative real-time PCR (qRT-PCR) was used to detect the expression of miRNA-181a and miRNA-373 in bone marrow tissues of each group. The differences in the expression of miRNA-181a and miRNA-373 between these groups and their correlation with clinicopathological features of MM were analysed. Pearson linear correlation analysis were used to show the correlation between miRNA-181a and miRNA-373 expression. Results Compared with the case control group and the healthy control group, the expression of miRNA-181a was significantly higher in the case group and the expression of miRNA-373 was significantly lower (P ＜ 0.05), but there was no difference in the expression of miRNA-181a and miRNA-373 between the case control group and the healthy control group (P ＞ 0.05). The expression of miRNA-181a and miRNA-373 in bone marrow tissue of MM patients was related to tumor stage and tumor differentiation (P ＜ 0.05), but not related to age, gender, cytological type and lymph node metastasis (P ＞ 0.05). There was a significant negative correlation between miRNA-181a and miRNA-373 expression in the bone marrow of the case group (r = -0.696, P ＜ 0.05). There was no significant correlation between the case control group and the healthy control group (r = 0.151, 0.179, P ＞ 0.05). Conclusion The expression of miRNA-181a is increased in MM bone marrow tissue, and the expression of miRNA-373 is decreased. Both of them participate in the tumor development and progression, which may become a new marker for diagnosis, treatment and prognosis of MM.
展开▼
