Objective To research and observe the influence of storage time and storage way of blood sample on blood glucose testing result, for providing reference for clinical detection in clinical practice. Methods 20 cases were selected randomly, and the patients were collected blood samples with empty belly. 14mL blood was collected for each patient, and 2mL once. The blood samples were stored in vacuum tubes which were marked as control(1), indoor temperature(2), 37℃thermostatic waterbath(2) and 0℃fridge(2). And the influence of storage time and storage way of blood sample on blood glucose testing result was analyzed. Results The influence of storage time of blood on blood glucose test is very evident. After 6h, the blood glucose level was 60%of the initial value. After 18h, he blood glucose level was 27%of initial value.And the blood glucose level after 24 h is 22%of initial value. The longer blood glucose was stored, the faster the blood glucose level reduced. After blood samples were stored in indoor temperature and 37℃thermostatic waterbath for 2.5h and 5h, the blood glucose testing result had evident difference from the initial value, which had statistical significance. Blood samples are stored in sodium fluoride potassium oxalate tube and separation gel coagulant tube, and the influence of it on blood glucose detection had no evident difference, which had no statistical significance. Conclusion In order to ensure that the result of blood glucose detection is accurate, the blood samples should be stored in the environment with low temperature, and the blood samples should be detected timely, to reduce the error of detection results and ensure that the result is reliable and accurate.%目的：研究和探讨血液标本存放时间和存放方式对血糖检测结果的影响，为日后临床检验提供参考价值。方法随机抽取20例患者，空腹采集血液标本，每位患者共采集14mL，每次2mL，存放于真空管中，并标注为对照（1个）、室温（2个）、37℃恒温水浴（2个）和0℃冰箱（2个），分析存放时间和存放方式对血糖检测结果的影响。结果血液存放时间对血糖测试的影响非常显著，6h后血糖值为初始值的60%，18h后血糖值为初始值的27%，24h后血糖值为初始值的22%，血糖存放时间越长，血糖值下降的速度越快。血液标本存放在室温和37℃恒温水浴2.5h和5h后血糖监测结果和初始值有显著差异，差异有统计学意义。氟化钠草酸钾管和分离胶促凝剂管保存血液样本，对血糖值检测的结果影响无明显差异，差异无统计学意义。结论为了确保血糖检测的结果准确，应当将血液标本保存在低温的环境下，并且及时对血液标本进行检测，以减少检验结果的误差，保证结果的可靠性和准确性。
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