采用经某地浸采铀矿山初步驯化的氧化亚铁硫杆菌（T ．f ），开展了地浸采铀溶液中共存的SO2－4、NO3－、Cl－、Zn2＋、Cu2＋、M n2＋、Cr3＋、U、F－等对T ．f活性影响和共存离子对 T ．f活性影响试验。结果表明：单一离子对T ．f活性影响大小为：F－＞ U＞ M n2＋＞Cr3＋＞Zn2＋＞Cl－＞ NO3－＞SO2－4。其中 T ．f对SO2－4、NO3－、Cl－、Zn2＋、Cu2＋、Mn2＋、Cr3＋的耐受浓度分别为32g ．L －1、3．2g ．L －1、3．2g · L －1、0．40 g·L －1、3．2g·L －1、0．40g·L －1、0．40g·L －1，且尚未达到最大耐受浓度；T．f 耐受 U 浓度为0．80 g·L －1，当U浓度为1．60g·L －1时T ．f失去活性或基本不能氧化 Fe2＋；F－对 T ．f活性影响最大，试验菌株对F－的耐受浓度仅为20mg · L －1。驯化后的细菌已在新疆某地浸矿山工业试验中得到应用。%Thiobacillus ferrooxodans (T .f) were domesticated in in‐situ leaching uranium mine and the effects test of coexisting ions such as SO2 -4 、NO3- 、Cl- 、Zn2+ 、Cu2+ 、Mn2+ 、Cr3+ 、U、F- etc .in solution on T .f activity have been investigated .The test results showed that the order of influence of different ions on bacteria activities was F- > U>Mn2+ >Cr3+ >Zn2+ >Cl- >NO3- >SO2 -4 and the tolerant concentrations to SO2 -4 、NO3- 、Cl- 、Zn2+ 、Cu2+ 、Mn2+ and Cr3+ were 32g · L -1 、3 .2g · L -1 、3 .2g · L -1 、0 .40g · L -1 、3 .2 g · L -1 、0 .40 g .L -1 and 0 .40g · L -1 respectively and the most tolerant concentrations of ions were not still obtained .The tolerant concentration to U was 0 .80g · L -1 .When the concentration of U was 1 .60g · L -1 , bacteria lost activities and could not oxide Fe2+ to Fe3+ .The bacteria activities were affected by F-intensively .The tolerant concentration to F- was only 20mg · L -1 .Domesticated bacteria have been used in field test of in‐situ uranium leaching in Xinjiang .
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