目的: 建立测定琥珀酸亚铁片中三价铁和二价铁含量的方法.方法: 采用Dionex RFICTM保护柱(4mm×50mm),Dionex RFICM Ion PacRCS5A分析柱(4 mm×250 mm), 淋洗液:7 mmol·L-1 吡啶二羧酸、66 mmol·L-1氢氧化钾、5.6 mmol·L-1硫酸二氢钾和74 mmol·L-1甲酸的混合溶液;流速:1.5 ml·min-1;柱温:30.0℃;进样体积:1.3 μl.结果: Fe3+在0.5~15 μg·ml-1范围内线性良好(r=1.000 0);Fe2+在25~200 μg·ml-1范围内线性良好(r=1.000 0);Fe3+的平均回收率为103.6%(RSD=2.7%, n=9);Fe2+的平均回收率为98.3%(RSD=1.9%, n=9).结论: 该方法简便可靠,结果准确,可用于琥珀酸亚铁片的含量测定.%Objective: To establish a content determination method for ferric iron and ferrous iron in ferrous succinate tablets.Methods: Using a Dionex RFICTM protection column (4 mm×50 mm)and a Dionex RFICM Ion PacRCS5A analytical column (4 mm×250 mm), the eluent solution consisted of 7 mmol·L-1 dipicolinic acid, 66 mmol·L-1 potassium hydroxide, 5.6 mmol·L-1 potassium phosphate monobasic and 74 mmol·L-1 formic acid.The flow rate was 1.5 ml·min-1, the column temperature was 30.0℃ and the injection volume was 1.3 μl.Results: Fe3+ showed good linearity within the range of 0.5-15 μg·ml-1(r=1.000 0), Fe2+ showed good linearity within the range of 25-200 μg·ml-1(r=1.000 0), and the average recovery was 103.6%(RSD=2.7%, n=9)and 98.3%(RSD=1.9%, n=9), respectively.Conclusion: The method is simple, reliable and accurate, and can be used for the determination of Fe3+and Fe2+ in ferrous succinate tablets.
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