In order to prepare hydrogel containing imidazole group,Im-PAA-HEAA macromer and polyethylene glycol diacrylate (PEGDA)were mixed homogeneously and then they were gelled to yield Im-PAA-HEAA/PEGDA hydrogel through radical reac-tion in UV radiation.Hydrogels show obvious volume shrinkage,water absorption reduction and mechanical enhancement in low concentration of zinc ion solution and exhibit excellent biocompatibility.A non-viral gene vectors Im-PEI which can combine DNA effectively and shows low cytotoxicity and high gene transfect efficiency is synthesized by grafting urocanic acid onto PEI.Due to the complexing force between the Zn2+ and imidazole,the Im-PEI/pDNA can be adsorbed on the surface of hydrogels with imid-azole.The results indicate that Im-PAA-HEAA/PEGDA hydrogels can absorb more Im-PEI/DNA complexes by zinc ions com-plexion than these without zinc ions.Hydrogels may have great potential in reverse gene transfection.%为了制备含有咪唑基团的水凝胶，本文将反应制备的 Im-PAA-HEAA 大分子单体和聚乙二醇二丙烯酸酯（PEGDA）混合，在紫外照射下发生自由基反应，从而得到 Im-PAA-HEAA／PEGDA 水凝胶。在低浓度的锌离子溶液刺激下，水凝胶表现出体积收缩、吸水率下降和强度增强等响应性，并且表现出良好的细胞相容性。将尿刊酸接枝于聚乙烯亚胺（PEI），制成含咪唑的非病毒基因载体（Im-PEI），该载体可以有效复合 pDNA，并具有低细胞毒性和较高的转染效率。利用锌离子和咪唑之间的络合作用，可以将 Im-PEI／DNA 复合物有效地固定于 Im-PAA-HEAA／PEGDA 水凝胶表面。研究结果表明：与无锌离子作用相比， Im-PAA-HEAA／PEGDA 水凝胶在锌离子作用下能够吸附更多的 Im-PEI／DNA 复合物，该水凝胶有望在反向基因转染方面得到应用。
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