A pal-RLRRLRARARA (referred to as p-RRL, pal is short for fatty acid whose molecular formula is C16H32O2) has been synthesized by means of the Fmoc solid-phase synthesis based on the glycoprotein GPIbβ C-terminal transmembrane sequence. The self-assembly of this peptide and the effect of environment on it were explored. The differences in microscopic arrangement under different conditions were demonstrated with CD, and the morphology of peptide hydrogel nanofiber was characterized by TEM and SEM. The results indicate that peptide p-RRL can self-assemble into hydrogel which is pH-sensitive; pH, solvent and temperature have different effects on the secondary structure of peptides, thereby affecting the self-assembly of this peptide; although the β-sheet structure is beneficial to gelation, not all the secondary structures of peptide which can self-assemble into hydrogel are of β-sheet structure.% 以糖蛋白GPIbβ跨膜区C-末端一段序列为基础,采用Fmoc固相合成法合成多肽pal-RLRRLRARARA(简称p-RRL,pal是脂肪酸的简写,分子式C16H32O2);考察了此多肽自组装性质以及不同环境对此多肽自组装的影响;采用圆二色谱测试了不同条件下多肽分子间的微观排列情况,并利用透射电镜和扫描电镜对形成的凝胶的形貌进行表征。研究发现多肽p-RRL可以自组装成pH敏感型超分子水凝胶; pH、溶剂以及温度均会对多肽的二级结构产生影响,进而影响多肽自组装;虽然β-折叠结构有利于成胶,然而并不是所有能够形成水凝胶的多肽二级结构均是β-折叠
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