In this work, enhanced Raman spectra of cytochrome c (Cyt c) absorbed on TiO2 nanoparticles have been observed. Raman spectra are quite different on Cyt c bulk according to the interaction between Cyt c and TiO2 nanoparticles. The positions of some peaks shift and the relative intensities of some peaks change as well. For the quantitative analysis, the concentration of Cyt c is adjusted from 10-4 mol/L to5×10-7 mol/L.Aconcentration-dependentrelationshipwithintensityisfound.Meanwhile,thedetectionlimitofCytccanachieveaslowas 5×10-6 mol/L.Itispossibletocarryoutsemi-quantitativeanalysisoftheconcentrationofCytc.% TiO2纳米粒子具有良好的光学活性、催化活性及生物相容性.在TiO2纳米粒子表面可观测到Cyt c分子增强的拉曼信号.同Cyt c本体的拉曼光谱对比得知,吸附在TiO2表面的Cyt c分子的光谱有所不同,某些峰发生位移,某些峰的相对强度发生变化.同时,调节Cytc的浓度变化范围从10-4 mol/L至5×10-7 mol/L,发现了Cytc分子增强拉曼光谱的浓度依赖性.吸附在TiO2纳米粒子表面的Cytc检测限可以达到5×10-6 mol/L.
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