Chitosan-poly (N-vinylpyrrolidone)-cysteine graft copolymer (CTS-g-PVP-cys) was prepared thanks to the excellent film-forming and the water retention of CTS,antioxidant and antimicrobial activities of L-cys and hydrophilic properties of PVP.The inhibitory effects of CTS-g-PVP-cys on Escherichia coli,Bacillus cereus and Staphylococcus aureus were observed via the method of inhibition zone.The experimental results indicate that:when the content of thiol in copolymer is 266 μmol/g,CTS-g-PVP-cys exerts good antimicrobial effect on Escherichia coli and Staphylococcus aureus and the maximum inhibition rate are 87.50％ and 89.33％ respectively,that is,the copolymer has coordinated substantially the antimicrobial activity of cys.CTS-g-PVP-cys films can also reduce the losses of nutrients and water in fruits,it can be used as a good fresh keeping agent for fruits anti-browning.%基于壳聚糖良好的成膜、保水性,结合半胱氨酸的抗氧化、抑菌性和N-乙烯基吡咯烷酮的亲水性,制备了壳聚糖-乙烯基吡咯烷酮-半胱氨酸接枝共聚物(CTS-g-PVP-cys).采用抑菌圈法研究了CTS-g-PVP-cys对大肠杆菌、金黄色葡萄球菌和蜡样芽孢杆菌的抑菌效果.结果表明:当共聚物中巯基含量为266 μmol/g时,CTS-g-PVP-cys对大肠杆菌、金黄色葡萄球菌均有较好抑菌效果,最大抑菌率分别为87.50％,89.33％,即CTS-g-PVP-cys很大程度上协同了半胱氨酸的抑菌性.同时,CTS-g-PVP-eys涂膜可降低水果中营养物质及水分的损失,具有防褐变效果,可作为良好的保鲜剂.
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