A high sensitive flow-injection chemiluminescence (FI-CL) method for the determination of tryptophan has been developed. The method is based on the chemiluminescence reaction of galangin-potassium permanganate-tryptophan in polyphosphoric acid (PPA) media. Under the optimized conditions, tryptophan was determined in the range 0.05-10 μmg/mL with the detection limit (3σ) of 5.0 ×10à3 μmg/mL. The relative standard deviation (RSD) was 1.0% for 11 replicate determinations of 1.0 μmg/mL tryptophan. Three synthetic samples were determined selectively with recoveries in the range from 99.6% to 102.0% in the presence of other amino acids.
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