In this paper, the adsorption process of calmodulin (CaM) on Au substrate was first investigated with electrochemical impedance spectroscopy (EIS) method. The result reveals that the adsorption of the protein-calmodulin contains two steps, i.e., one short quick step followed by a slow one. The complexation of calmodulin with Ca2+ was also first probed using EIS technique, in which the complexation of CaM with Ca2+ could be reflected by the change of apparent membrane capacitance(Capp) clearly. In all above measurements, a redox couple Fe(CN)63-/ Fe(CN)64- was used as probing-pin to reflect all the changes occurring in the above process. Our work suggests that some biological processes of CaM could be studied using EIS method conveniently.
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