Objective: To study the relationship between sterol regulatory element binding protein-1 (SREBP-1) and nucleotide-binding oligomerization domain-like receptor protein-1 (NLRP-1) inflammasome in patients with coronary artery disease (CAD). Methods: Our research included in 3 groups: Control group, n=20 normal subjects, SAP (stable angina pectoris) group, n=49 and ACS (acute coronary syndrome) group, n=55. Plasma levels of IL-1β, IL-18 and hs-CRP were examined by ELISA, mRNA and protein expressions of SREBP-1, NLRP-1 and Caspase-1 in peripheral blood mononuclear cells (PBMC) were detected by RT-PCR and Western blot analysis. Relationships between SREBP-1 and NLRP1, Caspase-1, IL-1β, IL-18 were studied. Results: Compared with Control group, ACS group and SAP group had increased plasma levels of IL-1β, IL-18 and hs-CRP, all P<0.05; elevated mRNA and protein expressions of SREBP-1, NLRP-1 and Caspase-1 in PBMC, in addition, those expressions in ACS group was even higher than SAP group, all P<0.05. SREBP-1 level was positively related to NLRP1, Caspase-1, IL-1β and IL-18, all P<0.05. Conclusion: The expressions of SREBP-1 and NLRP1 inflammasome were increased in CAD patients; SREBP-1 and NLRP1 had positive correlation.%目的:研究冠心病患者固醇调节元件结合蛋白-1(SREBP-1)与核苷酸结合寡聚化结构域样受体蛋白1(NLRP1)炎性体的关系.方法:研究对象分为3组,正常对照组(n=20)、稳定性心绞痛组(SAP组,n=49)和急性冠状动脉综合征组(ACS组,n=55).用酶联免疫吸附法检测血浆白细胞介素-1β(IL-1β)、IL-18和高敏C反应蛋白(hs-CRP)水平,实时荧光定量聚合酶链反应和蛋白免疫印迹分别检测外周血单个核细胞(PBMCs)内SREBP-1、NLRP1和半胱天冬酶(Caspase-1)的RNA和蛋白表达,分析SREBP-1表达水平与NLRP1、Caspase-1、IL-1β和IL-18的相关性.结果:ACS组和SAP组血浆IL-1β、IL-18表达和hs-CRP水平较正常对照组显著增高,且ACS组患者较SAP组显著增高,差异均有统计学意义(P均<0.05);ACS组和SAP组PBMCs内SREBP-1、NLRP1和Caspase-1的RNA和蛋白表达均较正常对照组显著增高,且ACS组患者较SAP组显著增高,差异均有统计学意义(P均<0.05);SREBP-1表达水平与NLRP1、Caspase-1、IL-1β和IL-18表达均呈正相关(P均<0.05).结论:冠心病患者SREBP-1与NLRP1炎性体表达升高,且二者呈正相关.
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