Background As the people living in the plateau move back and forth between the plateau and the plain more and more frequently,the adaptation of the plateau hypoxia population to the oxygen enriched environment of the plain has gradually attracted wide attention.Objective To analyze the serum protein expression in Tibetan and Han Chinese college students with unwell symptoms who migrated to the inland region of China from Tibet based on serum proteomics,in order to provide an accurate and simple method for early diagnosis of the diseases due to maladjustment in such a population,and to offer molecular targets for the related clinical treatment.Methods The participants met the inclusion criteria were 32 Tibetan and Han college students〔Tibetan group(n=16) and Han group(n=16)〕 with unwell symptoms who enrolled in Xizang Minzu University during the periods of October to November 2013,October to November 2014 and October to November 2015.Serum samples were collected and the serum protein-derived polypeptide was captured by magnetic bead-based weak cation-exchange chromatography(MB-WCX).The expression of serum proteins was analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) technique and compared between the two groups.Results MALDI-TOF-MS analysis of single sample found that,the polypeptide curves of serum protein with molecular weight of 1 000-10 000 m/z in the 3 samples from the same individual in the Tibetan group tended to be consistent,and similar results were obtained in the Han group;there were significant differences in serum protein levels between Tibetan group and Han group.The results of the MALDI-TOF-MS analysis of overall sample showed that there were significant differences in expression levels of serum protein with the molecular weight of 1 000-10 000 m/z between the Tibetan group and Han group;the analysis of its protein polypeptide components showed that a phenomenon of mutual aggregation and less overlap;there were significant differences in the peak expression of serum proteins with molecular weights of 905.65,1 062.10,1 078.11,2 138.79,2 313.40,4 094.67,4 172.89,4 213.96,5 910.71 m/z between Tibetan group and Han group(P<0.05).Conclusion There are some differences in the expression of serum proteins between Tibetan and Han college students with unwell symptoms after migration to the inland region of China from Tibet,and the differences are significant in the expression of serum proteins with molecular weights of 4 094.67,2 138.79 and 905.65 m/z,which can be used as a basis for further identification and localization of the sequences and genes of differential proteins.%背景 随着世居高原人群往返于高原与平原之间愈发频繁,高原低氧人群对平原富氧环境的适应逐渐引起人们的重视.目的 基于血清蛋白组学对移居内地的藏、汉族不适症状大学生的血清蛋白表达进行分析,以期为西藏移居内地的藏、汉族大学生因适应不良而引起的疾病提供简单易行的、精确的早期诊断指标,为其临床治疗提供分子靶标.方法 分别选取2013年10-11月、2014年10-11月、2015年10-11月西藏民族大学符合纳入标准的藏、汉族不适症状大学生32例为研究对象.按照研究对象民族,将其分为藏族组和汉族组,各16例.收集研究对象血清样本,采用弱阳离子磁珠(MB-WCX)捕获血清蛋白多肽,采用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术进行质谱分析并比较两组血清蛋白表达水平.结果 单样本质谱分析结果显示,3次重复取样,藏族组同一个体分子量为1 000~10 000 m/z的血清蛋白多肽曲线基本趋于一致,汉族组得出相似结果;藏族组与汉族组血清蛋白表达水平存在显著差异.整体样本质谱分析结果显示,藏族组与汉族组分子量为1 000~10 000 m/z的血清蛋白表达水平存在显著差异;对其蛋白多肽成分分析,则呈现出相互聚集、较少重合的现象;藏族组与汉族组分子量为905.65、1 062.10、1 078.11、2 138.79、2 313.40、4 094.67、4 172.89、4 213.96、5 910.71 m/z的血清蛋白表达峰值间存在显著差异(P<0.05).结论 西藏移居内地的藏、汉族不适症状大学生血清蛋白表达存在一定差异,其中分子量为4 094.67、2 138.79、905.65 m/z的血清蛋白表达差异较大,为进一步鉴定和定位差异性蛋白的序列和基因奠定了基础.
展开▼
