Objective ToinvestigatetheeffectofKudzuvineRootbloodcirculationpromotingdrink(KRBCPD)on regulating the expression of endoplasmic reticulum stress signaling pathway-related BIP mRNA and protein in rabbit atherosclero-sismodel.Methods Atotalof32healthyJapanesebig-earrabbitswereselectedasstudysubjects,therabbitsweredividedin-to four groups:the normal control group,the model group,the low-dose KRBCPD group and the high-dose KRBCPD group, 8 cases in each group. Cases in normal control group were fed with basic feed,cases in model group were fed with high cholesterol feed(1% cholesterol and 0. 02% methionine were added in basic feed),cases in low-dose KRBCPD were fed with high cho-lesterol feed and KRBCPD(5 g·kg-1 ·d-1 ),cases in high-dose KRBCPD were fed with high cholesterol feed and KRBCPD (10 g·kg-1 ·d-1 ). Nine weeks later,the superior segment of thoracic aorta was quickly obtained,the lesions in thoracic aor-ta were observed by form of organization observation method,the levels of BIP mRNA and protein which located in the cytoplasm ofVSMCinthoracicaortawasdetectedbyinsituhybridizationandWesternblottinghybridization,respectively.Results Mor-phological observation showed that vascular lesion in model group was typical,the AS model was successfully established. The vascular lesions in low-dose KRBCPD group and high -dose KRBCPD group got better,the repair effect in the high -dose KRBCPD group was better than that in the low-dose KRBCPD group. According to tissue in situ hybridization and Western blot-ting hybridization results,there were significant differences in levels of BIP mRNA and protein which located in the cytoplasm of VSMC in thoracic aorta among 4 groups(P<0. 05);The levels of BIP mRNA and protein in model group,low-dose KRBCPD group and high-dose KRBCPD group were significantly higher than those in the normal control group,the levels of BIP mRNA and protein in low-dose KRBCPD group and high-dose KRBCPD group were significantly lower than those in model group,the levels of BIP mRNA and protein in high-dose KRBCPD group were significantly lower than those in low-dose KRBCPD group (P<0.05).℅onclusion KRBCPDcanrepairHcy-inducedvascularinjury,theeffectofhigh-doseKRBCPDisbetterthan that of low-dose KRBCPD,the anti-AS mechanism of KRBCPD is correlated with KRBCPD's regulation effect on ERS signa-ling pathway-related BIP mRNA and protein.%目的：探讨葛根通脉饮对兔动脉粥样硬化（ AS）模型内质网应激（ ERS）信号转导通路免疫球蛋白重链结合蛋白（ BIP） mRNA及其蛋白表达的调控作用。方法选取健康日本大耳白兔32只，将其随机分为正常组、模型组、葛根通脉饮低剂量组（葛低组）、葛根通脉饮高剂量组（葛高组），每组8只。正常组饲喂基础饲料；模型组饲喂高胆固醇饲料（在基础饲料中加入1%胆固醇及0.02%蛋氨酸）；葛低组、葛高组在饲喂高胆固醇饲料的同时，分别给予葛根通脉饮5 g·kg-1·d-1和10 g·kg-1·d-1。饲喂9周后，取兔胸主动脉上段，采用组织形态观察法观察胸主动脉病变情况，采用组织原位杂交及Western blotting杂交方法分别检测胸主动脉血管平滑肌细胞（ VSMC）细胞质中BIP mRNA及其蛋白表达量。结果形态学观察显示，模型组血管病变典型，AS模型制备成功；葛低组、葛高组血管病变得到修复，其中葛高组血管修复效果明显。组织原位杂交及Western blotting杂交结果显示，4组兔胸主动脉VSMC BIP mRNA及其蛋白表达量间有差异（ P<0.05）；其中模型组、葛低组、葛高组BIP mRNA及其蛋白表达量高于正常组，葛低组、葛高组低于模型组，葛高组又低于葛低组（ P<0.05）。结论葛根通脉饮可拮抗由同型半胱氨酸（ Hcy）诱导的血管损伤，且高剂量的葛根通脉饮效果优于低剂量葛根通脉饮；葛根通脉饮抗AS作用机制与其对ERS通路BIP mRNA及其蛋白表达的调控有关。
展开▼
