Objective To explore the relationship between the polymorphism of the soluble epoxide hydrolase ( EPHX2 ) gene and the incidence of intracranial aneurysm in the Han nationality. Methods Two hundred and thirty eight patients with intracranial aneurysms and 221 controls were studied, the genotype and allelic frequency of the EPHX2 rs751141 and rs2291635 polymorphism were analyzed by polymerase chain reaction ( PCR ) and sequencing of the PCR products. Results EPHX2 rs751141 polymorphism was significantly different between the aneurysm group [ with a ( GA + AA ) genotype of 22. 2% ] and the control group [ with a ( GA + AA ) genotype of 41. 7% ] among females ( P =0. 002 ) . However, this association wasn' t found in males and in all subjects regardless of genders ( P > 0. 05 ) . No significant difference was seen in the genotype and allelic frequency of rs2291635 polymorphism between patients and controls in male, female or all subjects. Conclusion The rs751141 polymorphism of EPHX2 is correlated with the pathogenesis of intracranial aneurysms in the Han nationality.%目的 探讨汉族人中可溶性环氧化物酶(EPHX2)基因多态性与颅内动脉瘤的关系.方法 利用聚合酶链式反应(PCR)和PCR产物直接测序的方法检测EPHXZ,比较238例颅内动脉瘤(病例组)与221例无颅内动脉瘤(对照组)EPHX2基因rs751141、rs2291635多态性位点的基因型频率.结果 女性颅内动脉瘤组与对照组中EPHX2 rs751141多态性基因型分布比较,差异有统计学意义,即前者GA+AA基因型频率(22.2%)低于后者(41.7%)(P=0.002);但在男性和不分性别患者中该基因型频率分布差异均无统计学意义(P＞0.05).rs2291635位点的基因型频率分布在男性、女性、不分性别患者中差异均无统计学意义(P＞0.05).结论 汉族人的rs751141基因异质性可能与女性颅内动脉瘤的发生相关.
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