目的 探讨磷脂酰肌醇-3-羟基激酶(PI3K)抑制剂LYZ94002在肝细胞生长因子(HGF)促进结肠癌细胞SW620增殖、侵袭中所起的作用.方法 分别用噻唑蓝比色法(MTT)、流式细胞术及划痕实验检测结肠癌细胞增殖、细胞周期、凋亡及移行情况.结果 (1)MTT法结果显示,LY294002≥20 μmol/L实验组与对照组吸光度(OD)值比较,差异有统计学意义(P＜0.05).(2)流式细胞术结果显示,LY294002≥20 μmol/L实验组与对照组凋亡率及增殖指数比较,差异有统计学意义(P＜0.05).(3)划痕实验结果显示,LY294002≥40 μmol/L实验组与对照组移行距离比较,差异有统计学意义(P＜0.05).结论 PI3K信号通路抑制剂能够阻断HGF对人结肠癌细胞增殖、凋亡、移行的促进作用.%Objective To investigate the role of PI3K inhibitors ( LY294002 ) in the proliferation, apoptosis and migration of SW620 activated by HGF. Methods The proliferation of SW620 cells activated by HGF was assessed by MTT, while the apoptosis was examined by Flow Cytometry ( FCM ) and the migration was detected by Scratch test. Results ( 1 ) MTT test showed that LY294002≥20 μmol/L, and the difference of OD value between experiment group and control group was statistically significant ( P <0. 05 ). (2) Flow cytometry showed that LY294002 ≥20 μmol/L The differences of apoptosis rate and proliferation index between experiment group and control group were statistically significant ( P < 0. 05 ). (3) Scratch test showed that LY294002 ≥40 μmo]/L. The difference of migration of SW620 between experiment group and control group was statistically significant ( P <0. 05 ) . Conclusion PI3K/AKT signaling pathway can inhibit the activation of proliferation, apoptosis and migration of SW620 by HGF.
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