Identification and activity evaluation of Astragalus Radix from different germplasm resources based on specific oligosaccharide fragments

Shu-ying Li; Di Wang; Xian-rong Li; Xue-mei Qin; Yu-guang Du; Ke Li

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Identification and activity evaluation of Astragalus Radix from different germplasm resources based on specific oligosaccharide fragments

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Objective: Based on trifluoroacetic acid(TFA) hydrolysis, polyacrylamide gel electrophoresis(PAGE) and high performance thin layer chromatography(HPTLC) analysis, the carbohydrate responsible for immunomodulatory activity are used as quality indicators for Astragalus Radix(AR).Methods: In this study, 24 batches of AR from different germplasm resources were selected as the research object, and AR polysaccharides were extracted. PAGE and HPTLC methods were used to analyze the partial acid hydrolyzate of AR polysaccharides and obtain a series of saccharide fingerprints. The data were analyzed by principal component analysis to obtain the difference between AR from different germplasm resources.Results: The results showed that trisaccharide and tetrasaccharide could be used as differential fragments to distinguish AR of different cultivation methods;Disaccharides and trisaccharides can be used as differential fragments to distinguish different species of AR. The immunological activity analysis of the specific oligosaccharide fragment of AR showed that the specific oligosaccharide fragment of AR could promote the secretion of TNF-a, IL-1 b, IL-6, and NO in THP-1 cells in a concentration-dependent manner.Conclusion: Both PAGE and HPTLC methods can be used to evaluate AR from different germplasm resources. This study laid the foundation for the quality evaluation of AR medicinal herbs.

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《中草药：英文版》 |2021年第1期|P.33-42|共10页
作者
Shu-ying Li; Di Wang; Xian-rong Li; Xue-mei Qin; Yu-guang Du; Ke Li;
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作者单位

National Glycoengineering Research Center Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology Shandong University Qingdao 266237 China;

Modem Research Center for Traditional Chinese Medicine Shanxi University Taiyuan 030006 ChinaCollege of Chemistry and Chemical Engineering Shanxi University Taiyuan 030006 China;

Shanxi Jianshuo Food and Drug Research Institute Co. Ltd. Taiyuan 030000 ChinaShanxi Academy of Traditional Chinese Medicine Taiyuan 030000 China;

Modem Research Center for Traditional Chinese Medicine Shanxi University Taiyuan 030006 China;

Institure of Process Engineering Chinese Academy of Sciences Beijing 100190 China;

Modem Research Center for Traditional Chinese Medicine Shanxi University Taiyuan 030006 ChinaInstiture of Process Engineering Chinese Academy of Sciences Beijing 100190 China;

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正文语种 chi
中图分类中药学;
关键词
Astragalus Radix; germplasm resources; hydrolyzate; polysaccharide; saccharide fingerprints;

机译：黄芪;种质资源;水解产物;多糖;糖指纹;

Identification and activity evaluation of Astragalus Radix from different germplasm resources based on specific oligosaccharide fragments

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