目的：探讨绞股蓝皂苷对衰老小鼠皮肤的抗氧化保护作用。方法：60只小鼠随机分成3组，青年组（A）、老年对照组（B）和老年给药组（C）；A、B组灌胃生理盐水20ml/kg，C组灌胃绞股蓝皂苷提取液8g/kg，每天1次。30天后取皮肤组织，检测丙二醛（MDA）含量，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）活性。结果：与A组比较，B组、C组皮肤组织中MDA含量增加，SOD、CAT、GSH-Px活性降低（＜0.05）；与B组比较，C组皮肤组织中MDA含量减少，SOD、CAT、GSH-Px活性增加（＜0.05）。结论：灌胃绞股蓝皂苷可减轻衰老小鼠皮肤的氧化损伤，具有延缓小鼠皮肤衰老的作用。%Objective To study the antioxidative protection of Gypenosides in aged mices skin. Methods 60 mices were selected and randomly divided into 3 groups:young control group (group A),aged control group (group B) and aged dose group (group C). In group A and group B,normal saline (20ml/kg) was given intragastricly. Gypenosides extracts at doses of 8g/kg was administered intragastricly in group C,once a day. Skin tissue from treated mices were prepared for analysis 30 days after treatments. Relative level changes of MDA,SOD,CAT and GSH- Px were measured. Results Increases in MDA and decreases in SOD,CAT and GSH- Px were observed in both group B and group C as compared with group A ( <0.05). Compared with mices in group B, mices in group C showed significant decreases in MDA and increases in SOD,CAT and GSH- Px ( <0.05). Conclusion Intragastric administration of Gypenosides extracts to mices could alleviate the oxidative injury to aged skin,and therefore could delay the skin aging in mices.
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