Objective: To explore the effect of Angelica sinensis polysaccharide on expressions of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase (GSH-Px) in alcohol injured mouse livers and its significance.Methods: A mouse model of acute alcoholic liver injury was established by a one-time gavage of high concentration of alcohol.The general situation of the mice was then observed by intraperitoneal injection of Angelica sinensis polysaccharides for 6 days.The changes of MDA,SOD and GSH-Px in serum biochemistry were detected in different conditions.Changes of liver morphology and structure were observed with light microscope.Results: After alcoholic liver injury in mice,serum MDA increased significantly,and SOD and GSH-Px decreased significantly.At the same time,the liver tissue showed diffuse steatosis.After being treated with Angelica sinensis polysaccharide,along with the structure of the liver tissue repair,serum MDA decreased significantly,and SOD and GSH-Px increased.Conclusion: Angelica sinensis polysaccharide enable to protect the hepatocytes by blocking lipid peroxidation.And,serum MDA,SOD and GSH-Px could be used to biomarkers for hepatic injury,progression and outcomes.%目的:探讨当归多糖治疗小鼠乙醇性肝损伤中丙二醛(MDA)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)的变化及意义.方法:通过一次性高浓度乙醇灌胃建立小鼠乙醇性肝损伤模型,之后连续腹腔注射当归多糖6d,血清生化检测MDA、SOD及GSH-Px,光镜观察肝形态结构变化.结果:小鼠乙醇性肝损伤后,血清MDA显著增高,SOD及GSH-Px显著下降,同时肝组织结构呈弥漫性脂肪变性损伤.用当归多糖治疗后,伴随肝组织结构的修复,血清MDA显著下降,SOD及GSH-Px增高.结论:当归多糖可通过阻断膜脂质过氧化起到保护肝细胞的作用,血清MDA、SOD及GSH-Px水平的变化对判断肝的损害程度、病情发展、评估预后有一定的意义.
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