目的 检测良性前列腺增生(BPH)患者组织中蛋白表达的特点,了解有无与BPH相关的特异性蛋白表达.方法 通过双向电泳方法 所发现的检测BPH组织特异性蛋白表达,αs1-Casein进行定量免疫组化研究来验证该蛋白表达的特异性.结果 通过对正常前列腺组织,BPH及前列腺癌组织进行免疫组织化学染色,发现αs1-Casein在90%(20/22)的BPH组织中呈弱到强表达,而在正常前列腺组织中未见表达(0/10),在前列腺癌组织中的表达也不足10%(3/30).结论 αs1-Casein可能是一种BPH诊断的生物标记物.%Objective To analyze the characteristic of protein profile of benign prostatic hyperplasia (BPH) tissue and to further find the special proteins associated with BPH. Methods Protein profiles of human expressed prostatic secretion (EPS) samples obtained from BPH patients and normal males were comparatively analyzed by two-dimensional gel electrophoresis. In the resulting electrophoretograms, several differentially expressed proteins were identified. In this study, a distinct expressed protein αs1-casein was validated in BPH. Results Immunohistochemistry analysis showed that αs1-casein was mainly distributed in the epithelium of hyperplastic prostate. The level of the exression of αs1-casein in the tissue of BPH was found to be higher than that in prostatic carcinoma; whereas no asl-casein was detected in normal tissue.Western blotting analysis was also revealed same expression pattern of asl-casein was detected in BPH-EPS and BPH-free EPS. Conclusion asl-casein might be associated with BPH development and be used as a potential biological marker for the diagnosis of BPH.
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