The aim of this study was to investigate the effects of dietary vitamin D and calcium levels on production performance,serum biochemical parameters and organ indexes of male mink under the fixed ratio of calcium to phosphorus.One hundred and seventeen healthy male minks with (135±5) day-old with similar body weight were randomly divided into 9 groups with 13 replicates per group and 1 mink per replicate.The experiment was conducted with a 3 × 3 factorial design using a basal diet containing 2 300 IU · kg-1 vitamin D and 2.0％ Ca.The dietary ratio of calcium to phosphorus was fixed to 1.7.Nine kinds of diets were prepared in the experiment.The supplementary vitamin D levels were 0,2 000 and 4 000 IU · kg-1 of the diets,respectively,while supplementary Ca levels were 0％,0.4％,0.8％,respectively.Calcium and vitamin D lev els were 2.0％ versus 2 300 IU · kg-1,2.0％ versus 4 300 IU · kg-1,2.0％ versus 6 300 IU· kg-1,2.4％ versus 2 300 IU· kg-1,2.4％ versus 4 300 IU· kg-1,2.4％ versus 6 300 IU· kg-1,2.8％ versus 2 300 IU · kg-1,2.8％ versus 4 300 IU · kg-1,2.8％ versus 6 300 IU · kg-1.The test lasted for 60 days.Production performance,serum nitrogen metabolism indexes and fat metabolism indexes were measured,and organ index was calculated.The results showed that:1) When the calcium supplementation level was 0％,the skin length of mink significantly increased (P＜0.05).The vitamin D and calcium levels had no significant effect on the body weight,body length and fur quality of mink (P＞0.05).2)The serum TP concentration significantly increased(P＜0.05) and serum TG concentration significantly decreased (P＜0.05) when the addtion level of vitamin D was 2 000 IU · kg-1.When the supplementary level of calcium was 0.8％,the serum HDL concentration of mink significantly increased (P＜0.05),and the concentration of LDL significantly decreased (P＜0.01).3) 0.8％ calcium supplementation significantly increased mink heart index,liver index and spleen index(P＜0.05),vitamin D supplementation in 0-2 000 IU · kg-1 significantly increased mink kidney index(P＜0.01).In conclusion,under the conditions of this experiment,when the dietary supplementation is 0-2 000 IU · kg-1 vitamin D (total 2 300-4 300 IU · kg-1 vitamin D in diet),protein metabolism and kidney index of mink can be promoted;when the dietary supplementation is 0.8％ Ca(total 2.8％ Ca in diet),heart index,liver index and spleen index of mink can be promoted.%旨在探讨在钙磷比固定的条件下,饲粮维生素D和钙水平对雄性水貂生产性能、血清生化指标及脏器指数的影响.选取(135±5)日龄健康、体重相近的短毛黑水貂公貂117只,随机分成9组,每组13个重复,每个重复1只.试验采用3×3双因素随机试验设计,饲粮钙磷比固定为1.7,基础饲粮中维生素D水平为2 300 IU·kg-1,钙水平为2.0％.设3个维生素D添加水平,分别为0、2 000、4 000 IU· kg-1;3个钙添加水平,分别为0％、0.4％、0.8％,共配制9种试验饲粮.9种饲粮的钙与维生素D水平分别为2.0％与2 300 IU· kg-1、2.0％与4 300 IU· kg-1、2.0％％与6 300 IU·kg-1、2.4％与2 300 IU·kg-1、2.4％与4 300 IU·kg-1、2.4％与6 300 IU· kg-1、2.8％与2 300 IU· kg-1、2.8％与4 300 IU· kg-1、2.8％与6 300IU· kg-1.试验期为60 d.分别测定水貂生产性能、血清中氮代谢指标和脂肪代谢指标,并计算脏器指数.结果表明:1)饲粮钙添加水平为0％时,水貂皮长显著增加(P＜0.05).维生素D和钙水平对水貂末重、体长和毛皮品质均无显著影响(P＞0.05).2)维生素D添加水平为2 000 IU·kg-1时显著提高血清TP浓度(P＜0.05),显著降低水貂血清TG浓度(P＜0.05).钙添加水平为0.8％时,水貂血清HDL浓度显著提高(P＜0.05)、LDL浓度极显著降低(P＜0.01).3)0.8％钙添加水平显著提高水貂心脏指数、肝脏指数、脾脏指数(P＜0.05),0～2 000 IU·kg-1维生素D添加水平极显著提高水貂肾脏指数(P＜0.01).综合以上指标,在本试验条件下,得出当水貂饲粮中添加0～2 000 IU·kg-1维生素D,总维生素D含量为2 300～4 300 IU·kg-1时,能够促进水貂蛋白质代谢,提高水貂肾脏指数;当水貂饲粮中添加0.8％钙、饲粮中总钙含量为2.8％时,能够提高水貂心、肝、脾脏指数.
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