旨在探究PI3K/AKT信号通路在鹿茸干细胞[包括生茸区骨膜(antlerogenic periosteum,AP)与角柄骨膜(pedicle periosteum,PP)细胞]中所发挥的作用,以期为揭示哺乳动物器官发生和完全再生机制提供借鉴.本研究通过MTT分析、细胞周期检测、细胞骨架染色等方法研究了抑制PI3K/AKT信号通路后对鹿茸干细胞增殖、细胞黏附、细胞周期、细胞骨架和促血管形成作用的影响.结果发现:1)相对于PP细胞,PI3K/AKT信号通路在调控AP细胞增殖和维持细胞骨架方面具有更为重要的作用;2)AP细胞条件培养液具有明显的促血管形成作用,PP细胞条件培养液不具有明显促进血管形成的作用.试验结果初步表明:相对于鹿茸再生,PI3K/AKT信号通路在鹿茸发生过程中的调控作用更为重要.%Understanding the role of the PI3K/AKT pathway in regulating basic parameters of antler stem cells,include antlerogenic periosteum (AP) cells and pedicle periosteum (PP) cells,and provide valuable insights into the mechanisms underlying mammalian organ generation and regeneration.In the present study,we investigated the effects of the PI3K/AKT pathway on antler stem cell proliferation,adhesion,cell cycle,cytoskeleton and angiogenesis.Our results showed that:1) PI3K/AKT pathway had a greater influence on proliferation of the AP cells and played a more important role in maintaining the normal cell cytoskeleton than those of the PP cells;2) conditioned medium of the APCs stimulated tube formation,whereas the PPCs did not significantly do so.Our results suggest that PI3K/AKT pathway may play more important role in antler generation than regeneration.
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