The aim of this study was to investigate the effect of active immunization using inhibin o subunit mature peptide(roINH) with Montanide(MON) and Freund's (FA) adjuvants on ovarian development in Xinjiang Fine-wool sheep. 72 Sprague Dawley female rats of (9-10)-week-old were divided into 3 groups and actively immunized against the recombination fusion protein of Xinjiang Fine-wool sheep roINH with roINH+MON adjuvant (MON group) or roINH+FA adjuvant (FA group), or injected with physiological saline (control group), respectively. Each rat received a primary immunization and 2 booster immunization at 20-day interval. The results showed that, the antibody titer of MON group was significantly higher than that of FA group (P <0. 05), compared to controls, mean concentration of FSH was higher (P<0. 01) in the MON and FA groups, and there was a significant increase in the peak of P in MON and FA groups (P <0. 05), respectively. For the entire period of the study, mean concentration and peak of FSH and P were of no significant difference between the two immune groups (P>0. 05); mean concentration and peak of LH between the two immune groups had no significant difference(P>0. 05).There was no significant increase between MON and FA groups in the number of mature follicle (P>0. 05), which were both significant higher than that in control group (P<0. 05). Compared to FA group, the inflammatorial size was smaller and the inflammatory reaction was weaker in MON group. These results clearly demonstrate that active immunization against inhibin a subunit mature peptide in MON or FA adjuvants both can enhance immune effect, furthermore, the inflammatory reaction of MON adjuvant is less than that of FA adjuvant.%本研究采用新疆细毛羊抑制素成熟区序列重组融合蛋白(roINH)作为免疫原,以Montanide (MON)和Freund's (FA)作为佐剂,主动免疫SD大鼠,测定生殖激素及卵巢发育情况,评价佐剂效应.选用72只9～10周龄性成熟雌性SD大鼠,随机分为3组,分别皮下注射生理盐水(对照组)、roINH+ MON(MON组)和roINH+FA (FA组).各组大鼠每20 d免疫1次,连续免疫3次.结果,MON组产生的抗体滴度显著高于FA组(P＜0.05)；与对照组相比,注射roINH+MON和roINH+FA均可极显著提高大鼠血清FSH平均含量(P＜0.01),显著提高P峰值(P＜0.05),而FSH及P平均含量及峰值在2个免疫组间无显著差异(P＞0.05)；LH含量和峰值在2个试验组间无显著差异(P＞0.05); MON和FA组成熟卵泡数无显著差异(P＞0.05),但均显著高于对照组(P＜0.05)；MON组脓包直径及炎症反应小于FA组.结果表明,应用roINH作为免疫原并配以MON或FA佐剂免疫大鼠均能取得较好的免疫效果,且MON佐剂炎症反应小于FA佐剂.
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