In order to investigate the prevalence status of avian leukosis virus infection in Shandong province, 3882 serum samples, 2428 cloacal swab samples and 41 suspected-case samples were collected from different areas of Shandong province for respectively detection through methods of serology, pathology and etiology. The test results indicated that P27 antigen and ALV antibody were positive in all stains of layer chickens including grandparent flock, the p27 antigen and antibody's positive rates of ALV-A/B, ALV-J, REV were 19. 36％, 9. 29％, 5. 18％,13. 77％, respectively. The main groups getting disease were Commercial flock and parent flock,while there're also some Hy-Iine brown grandparent flock got disease. The pathology diagnosis indicates that the tumors mainly include myelocytoma (27/41) , hemangioma (7/41) , fibrosarcoma (2/41), leiomyosarcoma (2/41) and MD (5/41). Among the 41 samples, 33 samples were positive for ALV-J (80. 49％), and 22 samples were positive for MDV (53. 66％), the co-infection rate was 43. 9％. Among 17 virus stains isolated, the sequence homology of gp85 gene of ALV-J were ranged from 94. 0％ to 100％. The 17 isolated virus stains shared 94. 3％ to 98. 7％identity with HPRS-103, and shared low homology with other strains (84. 4％-96. 8％). These results indicated that ALV infection existed in all stains of layer chickens in Shandong province and co-infection with MDV, REV were occured. Myelocytoma and hemangioma were the main clinical symptoms of sick chickens.%为了解山东不同品系蛋鸡白血病流行情况,作者采集了山东各地区主要引进品系及地方品种鸡的血清3882份、棉拭子2428份和疑似病例41例,对采集样品分别进行了血清学、病理学及病原学检测.结果表明:包括祖代鸡在内的各品系蛋鸡群P27抗原阳性率为19.36%;ALV-A/B亚型抗体阳性率9.29%、ALV-J亚型抗体阳性率5.18%、REV抗体阳性率13.77%;发病鸡群主要是商品鸡群和父母代鸡群,海兰褐祖代鸡群也有发病;病理学诊断证明肿瘤类型主要为髓细胞瘤(27/41)、血管瘤或血管内皮细胞瘤(7/41)、纤维肉瘤(2/41)、平滑肌肉瘤(2/41)及马立克氏病(5/41);PCR检测结果显示,41份病料中有33份为ALV-J阳性(80.49%);22份为MDV阳性(53.66%);两者共感染率高达43.9%;从疑似病例分离到的17株ALV-J病毒gp85基因的同源性为94.0%～100%,与ALV-J原型株HPRS-103 gp85基因同源性为94.3%～98.7%;与其它已发表的分离毒株ALV-J gp85基因的同源性较低(84.4%～96.8%).调查结果表明,目前山东各品系蛋鸡群均存在ALV感染,其中以ALV-J为主,ALV-A和ALV-B同时存在,且存在与MDV、REV的混合感染,病鸡主要表现髓细胞瘤和血管瘤.
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