24 0 day-old male broilers were reared under four light treatments (n=6), white (400-760 nm), red (660 nm), green (560 nm) and blue (480 nm) by using LED (light-emitting diodes) as light sources. Light intensity was 15 lx at the height of birds' heads and scheduled for 23 h of light and 1 h of dark during the entire experiment. Using the method of immunohisto-chemistry, the distribution and expression of GnRH were shown in different light groups at 49 day of age. The results showed GnRH neurons and fibers were distributed in hypothalamus, thalamus and mesencephalon, and GnRH expression in nucleus preopticus periventricularis (POP), nucleus preopticus medialis (POM), nucleus periventricularis hypothalami (PHN), nucleus paraventricularis magnocellularis (PVN), nucleus ovoidalis (OV) and nucleus rotundus (ROT) in blue light group was higher than those of in red and green light groups. The results suggested that color lights could affect the expression and secretion of GnRH in hypothalamus and thalamus of avian.%24只刚出壳的雄性AA肉鸡分别在白(400～760 nm)、红(660 nm)、绿(560 nm)和蓝色(480 nm) LED灯下饲养7周,光照强度15 lx,光照制度23h∶1 h(L:D).49日龄时取脑,利用免疫组化的方法显示GnRH神经元和神经纤维的分布及比较各光色下GnRH神经元的表达强度.结果显示,GnRH神经元和神经纤维在下丘脑、丘脑和中脑均有分布,且蓝光组下丘脑的视前室周核、视前内侧核、室周核、室旁大细胞核以及丘脑的圆核和卵圆核GnRH神经元表达强度显著高于红、绿光组.结果表明,光色可影响家禽下丘脑和丘脑GnRH的表达与分泌.
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