Aim:To investigate the effect of BuShen Yi Jing decoction on rat left-ventricular myosin heavy chain(MHC)α and βgene expression change with aging. Methods:Wistar rats were randomly divided into three groups:untreated 8 and 24 months of aging,administration drug 24 months of aging. Rats were administration buShen Yi Jing decoction six months with 30 times adults human dose. Rats serum thyroxine(T4) and triiodothyronine(T3) levels using an radioimmunoassay(RIA) from clinic assay,RT-PCR method was used to evaluate α and βMHC mRNA expression. Results: T4 level was increased 1.24 times of senescent administration drug rats contrast to senility control rats (P<0.001)，αMHC mRNA enhanced 1.3 times (P<0.05) and β MHC mRNA decreased 74.32%(P<0.05). Conclusion: Bushen Yijing decoction had reversed effect on α and βMHC gene expression change with aging.%目的：研究延衰中药补肾益精方对大鼠衰老过程(8～24月龄)中左心室肌球蛋白重链(α、βMHC)基因转录 mRNA表达量的影响，进一步探讨补肾益方的延衰机理。方法：大鼠喂饲到18月龄时随机分成老年组和老年服药组，中药用量是成人用量的30倍，服药6个月。以8月龄大鼠作为成年对照组。大鼠处死后，以常规放免法测定血清甲状腺素T3和T4水平。用RT-PCR方法扩增三组动物左心室α、βMHC 基因的有关转录产物，以GAPDH扩增片段做相对定量。结果：老年服药组与老年组相比，T4增加到1.24倍(P<0.001)，α MHC mRNA增加到1.39倍(P<0.05)，βMHC mRNA下调到74.32%(P<0.05)。结论：延衰中药对大鼠衰老增龄中α、βMHC基因表达的衰老变化有逆转作用。
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