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首页> 中文期刊> 《中国癌症研究：英文版》 >PREPARATION AND IDENTIFICATION OF MONOCLONAL ANTIBODIES AGAINST THE EXTRACELLULAR DOMAIN OF INTEGRIN a6 SUBUNIT-THE SPECIFIC LAMININ RECEPTOR

PREPARATION AND IDENTIFICATION OF MONOCLONAL ANTIBODIES AGAINST THE EXTRACELLULAR DOMAIN OF INTEGRIN a6 SUBUNIT-THE SPECIFIC LAMININ RECEPTOR

         
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摘要

Objective: To prepare monoclonal antibody (McAb) against the Integrin a6 extracellular domain and identify its biological activities. Methods: Fusion-protein of integrin a6 extracellular domain (GST-IAGED) was expressed in E.coli. JM109 and used for immunizing BALB/C mice. The spleen cells from immunized mice were fused with SP2/0 cells and selectively cultured with HAT medium. ELISA and immunocytochemistry staining were used to select hybridomas. Results: One strain of hybridoma cells that secreted specific monoclonal antibody against integrin a6 extracellular domain was indentified. The immunoglobulin subclass of the McAb was IgG1. Conclusion: The McAb against the extracellular domain of integrin a6 was successfully prepared by using GST-IA6ED fusion protein expressed by E.Coli. And the McAb had positive reaction with human hepatocarcinoma cells-BEL-7402.

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  • 来源
    《中国癌症研究：英文版》 |2002年第2期|137-140|共4页
  • 作者

    吕天敬; 张青云; 周柔丽;

  • 作者单位

    Department of Immunology;

    Peking University;

    School of Oncology;

    Beijing 100034;

    Department of Cell Biology;

    Peking University School of Basic Medical Science;

    Beijing 100083;

    Department of Immunology;

    Peking University;

    School of Oncology;

    Beijing 100034;

    Department of Cell Biology;

    Peking University School of Basic Medical Science;

    Beijing 100083;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 肿瘤的转移与扩散;
  • 关键词

    Integrin; Laminin; receptor; Hybridoma; McAb;

    机译：整合素;层粘连蛋白受体;杂交瘤;McAb;
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