Objective: TO understand the role of mitochondrialDNA (mtDNA) in carcinogenesis. Methods: single-stepmethod was used to isolate the mtDNA from humanlung adenocarcinoma cell line SPC-A-1. The mtDNAwas analyzed by restriction fragment lengthpolymorphism (RFLP) with 11 kinds of restrictionendonuclease, which were Pvu II, Xho I, Pst I, EcoR I,BstE II, Hind III, Hpa i, Bcl i, EcoR V, Sea I and Xba I.Restriction map of mtDNA from SPC-A-1 cell wasobtained by the single and double-digestion method.Results: It was found that no variation at 32 restrictionsites could be detected in the coding region of mtDNAfrom SPC-A-1 cell line. But a new site was found atnucleotide 16276 (EcoR V) within the noncoding region.Conclusion: These results indicate that the primarystructure of gene coding region of mtDNA isolated fromSPC-A-1 cell is highly stable. While the major variationof nucleotide is probably located in the noncodingregion.
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