Manganese peroxidase production from cassava residue by Phanerochaete chrysosporium in solid state fermentation and its decolorization of indigo carmine

Huixing Li; Ruijing Zhang; Lei Tang; Jianhua Zhang; Zhonggui Mao

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Manganese peroxidase production from cassava residue by Phanerochaete chrysosporium in solid state fermentation and its decolorization of indigo carmine

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Bioconversion of lignocellulosic wastes to higher value products through fungal fermentation has economic and ecological benefits. In this study, to develop an effective strategy for production of manganese peroxidase (MnP) from cassava residue by Phanerochaete chrysosporium in solid state fermentation, the stimulators of MnP produc-tion were screened and their concentrations were optimized by one-at-a-time experiment and Box–Behnken design. The maximum MnP activity of 186.38 nkat·g−1 dry mass of the sample was achieved after 6 days of fer-mentation with the supplement of 79.5 mmol·L−1·kg−1 acetic acid, 3.21 ml·kg−1 soybean oil, and 28.5 g·kg−1 alkaline lignin, indicating that cassava residue is a promising substrate for MnP production in solid state fermen-tation. Meanwhile, in vitro decolorization of indigo carmine by the crude MnP was also carried out, attaining the ratio of 90.18%after 6 h of incubation. An oxidative mechanism of indigo carmine decolorization by MnP was pro-posed based on the analysis of intermediate metabolites with ultra-high performance liquid chromatography and gas chromatography tandem mass spectrometry. Using the crude MnP produced from cassava residue for indigo carmine decolorization gives an effective approach to treat dyeing effluents.

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《中国化学工程学报（英文版）》 |2015年第1期|227-233|共7页
作者
Huixing Li; Ruijing Zhang; Lei Tang; Jianhua Zhang; Zhonggui Mao;
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The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;

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Manganese peroxidase production from cassava residue by Phanerochaete chrysosporium in solid state fermentation and its decolorization of indigo carmine

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