目的 观察癌基因PIK3CA、PIK3CB在大肠癌组织中的表达及其相互关系,并分析PIK3CA和PIK3CB的表达对大肠癌患者预后的影响.方法 采用免疫组化EnVision法染色并结合图像进行分析,探讨PIK3CA和PIK3CB在大肠癌组织及正常大肠黏膜组织中的表达;应用RT-PCR和Western blot技术分别检测PIK3CA mRNA和PIK3CB mRNA及其相应蛋白的表达;回访125例大肠癌术后患者,生存分析用Kaplan-Meier法,生存曲线用Log-Rank检验.结果 在300例大肠癌蜡块组织及正常黏膜组织中,PIK3CA表达的平均光密度值分别为:10.087 9±2.148 7、6.937 6±2.065 3,PIK3CB表达的平均光密度值分别为:15.870 6±2.877 7、8.693 2±2.442 4.PIK3CA和PIK3CB在大肠癌组织中的表达具有相关性(r=0.68,P<0.05);RT-PCR检测结果显示,与正常大肠黏膜组织相比,大肠癌组织中PIK3CA mRNA和PIK3CB mRNA的表达水平均明显升高,且二者表达结果呈正相关(r=0.74,P<0.05);Western blot法检测发现PIK3CA和PIK3CB蛋白表达水平同样升高,二者表达结果呈正相关(r=0.71,P<0.05);生存分析显示PIK3CA和PIK3CB高表达与患者死亡风险具有明显相关性.结论 PIK3CA和PIK3CB在大肠癌组织中高表达,与正常黏膜组织中的表达相比差异较明显,且PIK3CA和PIK3CB在大肠癌的发生、发展中具有协同作用,PIK3CA、PIK3CB高表达亦是大肠癌患者预后相关的因素之一.检测大肠癌患者PIK3CA和PIK3CB的表达对于控制大肠癌的进展、确定大肠癌的靶点治疗及判断预后具有重要意义.%Purpose To investigate the expression and significance of PIK3CA and PIK3CB in human colorectal carcinoma, and study the correlation between expression of PIK3CA and PIK3CB and the prognosis of colorectal carcinoma. Methods Immunohistochemical and image analysis were to detect the expression of PIK3CA and PIK3CB in tumor tissue and normal colorectal mucosa tissue. RT-PCR and Western blot were employed to detect the expression of PIK3CA and PIK3CB. After the follow-up results were obtained for 125 colorectal cancer patients, survival analysis was used by Kaplan-Meier methods and survival curve was examined by Long-Rank test. Results Of 300 cases of colorectal carcinoma and normal colorectal mucosa, average value of light density of PIK3CA was 10. 087 9 ?2. 148 7 and 6. 937 6 ?2. 065 3, PIK3CB was 15. 870 6 ?2. 877 7 and 8. 693 2 ?2. 442 4 respectively. The expression of PIK3CA and PIK3CB were a linear correlation and the correlation coefficient ( r ) of them was 0. 68. There was a positive correlation between the expression of PIK3CA and PIK3CB in colorectal carcinoma. The expression of PIK3CA and PIK3CB mRNA and protein in tumor tissue were higher than those in normal colorectal mucosa tissue. There was a positive correlation between the expression of PIK3CA and PIK3CB in tumor tissue, and the correlation coefficient ( r )of them were 0. 74 and 0. 71 respectively. Kaplan-Meier survival analysis showed that the patients with high PIK3CA expression and high PIK3CB expression in the tumors had a lower survival rate ( P <0. 05 ). Conclusion The results suggest that PIK3CA and PIK3CB have a coordinated effect in the carcinogenesis and development in human colorectal carcinoma, and further to supply experimental and theoretical guides for controlling the progression of colorectal carcinoma, determining the therapeutic target for colorectal carcinoma and evulating the prognosis of colorectal carcinomas.
展开▼
