Purpose To investigate the expression of Sema4D and vascular endothelial growth factor 2 ( VEGFR2 ) in colorectal adeno-carcinoma,and to explore their clinical significance. Methods The expression of Sema4D and VEGFR2 was determined by tissue mi-croarray and immunohistochemical staining in 115 cases of colorectal adenocarcinoma, 19 cases of colorectal adenoma and 12 cases of normal mucosa. The relationship between their expressions and the clinicopathologic parameters of the patients was analyzed. Results The positive expression of Sema4D in normal mucosa was 16. 67% , which had significant differences from 31. 58% in adenoma and 52. 17% in cancer specimens, and Sema4D was closely correlated to the Dukes stage and lymph node status( P <0. 05 ). The positive expression of VEGFR2 in normal mucosa was 16. 67% , which had significant differences from 31. 58% in adenoma and 52. 17% in cancer specimens, and VEGFR2 was closely correlated to the Dukes stage, invasion grade and lymph node status( P <0. 05 ). Conclusion Sema4D and VEGFR2 may play an important role in the development of colorectal adenocarcinoma.%目的 探讨Sema4D和血管内皮生长因子受体2(VEGFR2)在大肠腺癌组织中的表达及其临床意义.方法 运用组织芯片和免疫组化SP法检测115例大肠腺癌组织、19例大肠腺瘤组织和12例正常大肠黏膜组织中Sema4D与VEGFR2的表达,并分析其与临床病理特征的关系.结果 Sema4D在正常大肠黏膜表达阳性率8.33%,在腺瘤和腺癌中阳性表达率为15.79%和39.13%,差异有统计学意义且与淋巴结转移、Dukes临床分期明显相关(P<0.05).VEGFR2在正常大肠黏膜中阳性率16.67%,在腺瘤和腺癌中表达率为21.05%和51.3%,差异有统计学意义其表达与淋巴结转移、浸润程度、Dukes临床分期明显相关(P<0.05).结论 大肠腺癌中Sema4D与VEGFR2的表达可能在大肠腺癌的发生、发展中发挥重要作用.
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