Purpose To investigate the expression and clinical significance of DOG1 and WISP-1 in gastrointestinal stromal tumors ( GISTs ). Methods One hundred and twenty - four GISTs with complete clinicopathologic data were retrieved from the archival files of the department of pathology, Affiliated Hospital of North Sichuan Medical College. The expression levels of DOG1 and WISP-1 were detected by immunohistochemistry in 124 cases of GIST tissues, and specimens from non-GISTs were used as controls. Statistical analysis were performed using x2 test and Spearmen test. Result Of 124 GISTs, 119( 96％ ) were positive for DOG1 and 111 ( 91.0％ )for CD117. DOG1 protein expression rate was significantly different between GISTs and non-GISTs ( P ＜ 0. 001 ), but there were no significant differences between DOG1 and CD117 expression in GISTs. As for WISP-1 , this antibody yielded positive staining in 100 0f 124 ( 80. 6％ ) GISTs. The expression rate of WISP-1 protein in very low risk, low risk, intermediate risk, and high risk cases was 44. 4％( 4/9 ),66. 7％( 26/39 ),86. 9％ ( 17/20 ), 95. 0％ ( 38/40 ), and there was a significant correlation between WISP-1 protein expression and the risk score proposed by NIH( 2008 ). Conclusions DOG1 is a sensitivity and specificity marker for GIST and should be used as " the first line" antibody as CD117 protein in routine diagnosis. The expression of WISP - 1 maybe associated with GIST progression and could be used as a prognosis marker in GIST.%目的 探讨DOG1和WISP-1在胃肠道间质瘤(gastrointestinal stromal tumors,GIST)中诊断及预后的意义.方法 收集川北医学院附属医院病理科124例有完整临床病理资料的GIST,应用免疫组化EnVision两步法检测了DOG1和WISP-1蛋白在124例GIST中的表达情况,并与非GIST进行对照研究;应用χ2检验及Spearmen检验对结果进行统计学分析.结果 124例GIST中DOG1的阳性表达率为96%(119/124),CD117的阳性变表达率为91.0%(111/124),两者在GIST中的表达水平差异无统计学意义(P>0.05);GIST与非GIST中DOG1的表达水平差异有统计学意义(P0.05).结论 DOG1是GIST中一个敏感又特异的标记物,与CD117联用能提高GIST的诊断准确率,临床上可将其作为鉴别消化道间叶源性肿瘤的一线抗体;WISP-1的表达可能和GIST恶性进程有关,有可能作为评估GIST生物学行为的指标.
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