目的 研究吗啡受体在心肌腺苷预处理心肌保护中的作用.方法 以离体大白鼠工作心脏模型,比较经腺苷预处理和纳洛酮+腺苷预处理,在心肌缺血再灌前后,左室压(LVP)、左室舒张末期压(LVDEP)、左心室内压上升及下降最大速率(±dp/dtmax) 、主动脉压(AP)、冠脉流量(CF)、主动脉流量(AF)和测定冠脉流出液乳酸脱氢酶(LDH),心肌三磷酸腺苷(ATP)含量、超氧化物岐化酶(SOD)活性、脂质过氧化物(LPO)含量及自灌注停搏液至完全停搏的时间(AT).结果 复跳30 min后,除LVDEP 外,Control组和Nal+APT组各项心功能指标均低于ATP组,均差异有统计学意义(P<0.05).复灌工作心30 min后,各组LPO、LDH均有提高,但APT组明显低于于Nal+APT组和Control组;各组ATP、SOD均有所下降.结论 腺苷预处理的心肌保护作用需要阿片样受体介导,腺苷受体的心肌保护作用与阿片样受体可能存在交互作用.%Objective To investigate the role of opioid receptor on myocardial preservation with adenosine pretreatment in isolated rat hearts which suffer from ischemia/reperfusion injury.Method The model of Langendorff perfused isolated rat heart was used to study the effects of APT and Nal+APT on left ventricular pressure(LVP)、left ventricular end-diastolic pressure(LVDEP)、the maximal rate of rise and fall in left ventricular pressure (±dp/dt max)、aortic pressure(AP)、coronary flow(CF)、aortic flow(AF),activities of lactate dehydrogenase (LDH),superoxide dismutase(SOD) a lipid peroxidation(LPO) ,concentrations of ATP level pre-and post-ischemia/reperfusion.Result Nal+APT Compared with ischemia/reperfusion group and APT,Naloxone restrained the the recovery of cardiac hemodynamics and promoted the recovery of contractile function,decreased ATP level and SOD activity ,and increased leakage of intracellular lactic dehydrogenase.Conclusion Adenosine pretreatment cardioprotection requires the activation of opioid receptors and relies on these receptors functionally interacting with adenosine receptors.
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